Detection of QTLs for cold tolerance of rice cultivar ‘Kuchum’ and effect of QTL pyramiding

KEY MESSAGE : A QTL for cold tolerance at the booting stage of rice cultivar ‘Kuchum’ was detected and delimited into a 1.36 Mb region, and a cold-tolerant line was developed by QTL pyramiding. Low temperature in summer causes pollen sterility in rice, resulting in a serious loss of yield. The secon...

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Published in:	Theoretical and applied genetics Vol. 129; no. 3; pp. 631 - 640
Main Authors:	Endo, Takashi, Chiba, Bunya, Wagatsuma, Kensuke, Saeki, Kenichi, Ando, Tsuyu, Shomura, Ayahiko, Mizubayashi, Tatsumi, Ueda, Tadamasa, Yamamoto, Toshio, Nishio, Takeshi
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2016 Springer Springer Nature B.V
Subjects:	Adaptation, Physiological - genetics Agricultural production Agriculture Alleles Biochemistry Biomedical and Life Sciences Biotechnology Breeding Cold Cold Temperature Crosses, Genetic Cultivars Fertility Genetic aspects Genetic Markers Genotype Life Sciences Observations Original Article Oryza - genetics Plant Biochemistry Plant Breeding/Biotechnology Plant Genetics and Genomics Quantitative Trait Loci Rice Water treatment Japan INW, Japan Cold Stress Pyramid Cold Tolerance Culm Length Seed Fertility
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Summary:	KEY MESSAGE : A QTL for cold tolerance at the booting stage of rice cultivar ‘Kuchum’ was detected and delimited into a 1.36 Mb region, and a cold-tolerant line was developed by QTL pyramiding. Low temperature in summer causes pollen sterility in rice, resulting in a serious loss of yield. The second most widely grown rice cultivar in Japan, ‘Hitomebore’, has been developed as a cultivar highly tolerant to low temperature at the booting stage. However, even ‘Hitomebore’ exhibits sterility at a temperature lower than 18.5 °C. Further improvement of cold tolerance of rice is required. In the present study, QTLs for cold tolerance in a Bhutanese rice variety, ‘Kuchum’, were analyzed using backcrossed progenies and a major QTL, named qCT-4, was detected on chromosome 4. Evaluating cold tolerance of seven types of near isogenic lines having ‘Kuchum’ alleles around qCT-4 with a ‘Hitomebore’ genetic background, qCT-4 was delimited to a region of ca. 1.36 Mb between DNA markers 9_1 and 10_13. Homozygous ‘Kuchum’ alleles at qCT-4 showed an effect of increasing seed fertility by ca. 10 % under cold-water treatment. Near isogenic lines of ‘Hitomebore’ having ‘Silewah’ alleles of Ctb1 and Ctb2 and a ‘Hokkai PL9’ allele of qCTB8 did not exhibit higher cold tolerance than that of ‘Hitomebore’. On the other hand, a qLTB3 allele derived from a Chinese cultivar ‘Lijiangxintuanheigu’ increased cold tolerance of ‘Hitomebore’, and pyramiding of the qCT-4 allele and the qLTB3 allele further increased seed fertility under cold-water treatment. Since NILs of ‘Hitomebore’ with the ‘Kuchum’ allele of qCT-4 were highly similar to ‘Hitomebore’ in other agronomic traits, the qCT-4 allele is considered to be useful for developing a cold-tolerant cultivar.
Bibliography:	http://dx.doi.org/10.1007/s00122-015-2654-2 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s00122-015-2654-2