genes encoding chloroplast ribosomal proteins S7 and S12 are located in the inverted repeat of Spirodela oligorhiza chloroplast DNA

We have used a variety of methods to localize the genes for ribosomal proteins S7 and S12 on Spirodela chloroplast DNA. Heterologous hybridization with a rps12 gene specific probe from Euglena has revealed the presence of rps12 homologous sequences within the inverted repeat of Spirodela chloroplast...

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Bibliographic Details
Published in:	Current genetics Vol. 11; no. 1; pp. 25 - 34
Main Authors:	Posno, M, Verweij, W.R, Dekker, I.C, Waard, P.M. de, Groot, G.S.P
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-01-1986 Berlin
Subjects:	Agronomy. Soil science and plant productions Amino Acid Sequence Base Sequence Biological and medical sciences chloroplasts Chloroplasts - metabolism chromosome mapping Classical and quantitative genetics. Population genetics. Molecular genetics DNA DNA - genetics DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology gene expression Generalities. Genetics. Plant material Genes Genes. Genome Genetics and breeding of economic plants Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences Plants - genetics proteins Repetitive Sequences, Nucleic Acid Ribosomal Proteins - genetics ribosomes Spirodela oligorrhiza Genetic mapping Monocotyledones Vegetals Lemnaceae Chloroplast DNA Gene expression Ribosomal protein Molecular assembly Light Angiospermae Spermatophyta Inverted repeated sequence Chloroplast
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Summary:	We have used a variety of methods to localize the genes for ribosomal proteins S7 and S12 on Spirodela chloroplast DNA. Heterologous hybridization with a rps12 gene specific probe from Euglena has revealed the presence of rps12 homologous sequences within the inverted repeat of Spirodela chloroplast DNA on the fragment BamHI-V. In the partial nucleotide sequence of this fragment, two regions of amino acid sequence homology to Euglena S12 can be identified, separated from each other by a 542 bp intron with conserved boundary sequences. As was found for Nicotiana S12, the Spirodela S12 coding regions are for 85 amino acids homologous (79%) to E. coli S12 (starting from residue 38 to the C-terminus). Likewise, we are unable to identify the 37 5' terminal codons of rps12 in Spirodela. The functionality of the Spirodela rps12 sequence is discussed. The rps7 gene is located adjacent to rps12. Chloroplast ribosomal protein C-S11 (homologous to S7) has been detected by immunoprecipitation with both a polyspecific anti 30S serum and an anti C-S11 serum, among the in vitro translation products of mRNAs selected by Spirodela chloroplast DNA fragments BamHI-V and BamHI-P. Since in a DNA dependent E. coli cell free system, only BamHI-V appears to be capable of synthesis of C-S11, it is concluded that rps7 is located entirely within BamHI-V and is transcribed into a mRNA which extends into BamHI-P. As determined by Northern hybridization experiments, rps7 is cotranscribed with rps12; a stable transcript of approx. 1100 b is detected in total cellular Spirodela RNA with either rps12 and rps7 gene specific probes. The rps12 probe additionally detects an approx. 600 b transcript, which presumably corresponds to the excised rps12 intron RNA. Finally we have examined the expression of both rps7 and rps12 during light induced chloroplast development by Northern blotting and by immunoblotting. It is shown, that the steady-state levels of neither chloroplast ribosomal protein transcripts, nor those of the chloroplast ribosomal proteins itself, change significantly during the greening process.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0172-8083 1432-0983
DOI:	10.1007/BF00389422