Search Results - "WURM, Florian M"

Production of recombinant protein therapeutics in cultivated mammalian cells by Wurm, Florian M

“…Cultivated mammalian cells have become the dominant system for the production of recombinant proteins for clinical applications because of their capacity for…”
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Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors by De Jesus, Maria, Wurm, Florian M.

“…Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today’s technical status and to highlight emerging…”
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Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines by Balasubramanian, Sowmya, Rajendra, Yashas, Baldi, Lucia, Hacker, David L., Wurm, Florian M.

“…ABSTRACT Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in…”
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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions by Backliwal, Gaurav, Hildinger, Markus, Chenuet, Sebastien, Wulhfard, Sarah, De Jesus, Maria, Wurm, Florian M

“…Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low…”
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25 years of recombinant proteins from reactor-grown cells — Where do we go from here? by Hacker, David L., De Jesus, Maria, Wurm, Florian M.

“…The purpose of this review is to describe the current status and to highlight several emerging trends in the manufacture of recombinant therapeutic proteins in…”
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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives by Baldi, Lucia, Hacker, David L, Adam, Myriam, Wurm, Florian M

“…The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the…”
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Boosted Pro-Inflammatory Activity in Human PBMCs by Lipopolysaccharide and SARS-CoV-2 Spike Protein Is Regulated by α-1 Antitrypsin by Tumpara, Srinu, Gründing, Anna R., Sivaraman, Kokilavani, Wrenger, Sabine, Olejnicka, Beata, Welte, Tobias, Wurm, Maria J., Pino, Paco, Kiseljak, Divor, Wurm, Florian M., Janciauskiene, Sabina

“…For the treatment of severe COVID-19, supplementation with human plasma-purified α-1 antitrypsin (AAT) to patients is currently considered. AAT inhibits host…”
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Alpha1-antitrypsin improves survival in murine abdominal sepsis model by decreasing inflammation and sequestration of free heme by Zemtsovski, Jan D, Tumpara, Srinu, Schmidt, Sonja, Vijayan, Vijith, Klos, Andreas, Laudeley, Robert, Held, Julia, Immenschuh, Stephan, Wurm, Florian M, Welte, Tobias, Haller, Hermann, Janciauskiene, Sabina, Shushakova, Nelli

“…Excessive inflammation, hemolysis, and accumulation of labile heme play an essential role in the pathophysiology of multi-organ dysfunction syndrome (MODS) in…”
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Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures by Backliwal, Gaurav, Hildinger, Markus, Kuettel, Ivan, Delegrange, Fanny, Hacker, David L., Wurm, Florian M.

“…Various DNA methyl transferase inhibitors (iDNMTs) and histone deacetylase inhibitors (iHDACs) were screened for their ability to enhance transient gene…”
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Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells by Blessing, Daniel, Vachey, Gabriel, Pythoud, Catherine, Rey, Maria, Padrun, Vivianne, Wurm, Florian M., Schneider, Bernard L., Déglon, Nicole

“…Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during…”
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Affordable SARS-CoV-2 protein vaccines for the pandemic endgame by Triccas, James A., Kint, Joeri, Wurm, Florian M.

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Designs and Characterization of Subunit Ebola GP Vaccine Candidates: Implications for Immunogenicity by Agnolon, Valentina, Kiseljak, Divor, Wurm, Maria J, Wurm, Florian M, Foissard, Charlotte, Gallais, Fabrice, Wehrle, Sarah, Muñoz-Fontela, César, Bellanger, Laurent, Correia, Bruno Emanuel, Corradin, Giampietro, Spertini, François

“…The humoral responses of Ebola virus (EBOV) survivors mainly target the surface glycoprotein GP, and anti-GP neutralizing antibodies have been associated with…”
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Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor by Zhu, Likuan, Monteil, Dominique T., Wang, Yukui, Song, Boyan, Hacker, David L., Wurm, Maria J., Li, Xiaobin, Wang, Zhenlong, Wurm, Florian M.

“…[Display omitted] •Fluid dynamics in the OSR600 were studied by a CFD method.•The CFD model was validated by liquid wave imaging of the OSR600.•The energy…”
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The Delivery of α1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore by Tumpara, Srinu, Martinez-Delgado, Beatriz, Gomez-Mariano, Gema, Liu, Bin, DeLuca, David S., Korenbaum, Elena, Jonigk, Danny, Jugert, Frank, Wurm, Florian M., Wurm, Maria J., Welte, Tobias, Janciauskiene, Sabina

“…Human α1-antitrypsin (AAT) is an abundant acute phase glycoprotein expressing anti-protease and immunomodulatory activities, and is used as a biopharmaceutical…”
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Numerical simulation of orbitally shaken viscous fluids with free surface by Discacciati, Marco, Hacker, David, Quarteroni, Alfio, Quinodoz, Samuel, Tissot, Stéphanie, Wurm, Florian M.

“…SUMMARY Orbitally shaken bioreactors are an emerging alternative to stirred‐tank bioreactors for large‐scale mammalian cell culture, but their fluid dynamics…”
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Human PBMCs Form Lipid Droplets in Response to Spike Proteins by Sivaraman, Kokilavani, Pino, Paco, Raussin, Guillaume, Anchisi, Stephanie, Metayer, Charles, Dagany, Nicolas, Held, Julia, Wrenger, Sabine, Welte, Tobias, Wurm, Maria J, Wurm, Florian M, Olejnicka, Beata, Janciauskiene, Sabina

“…Intracellular lipid droplets (LDs) can accumulate in response to inflammation, metabolic stresses, and other physiological/pathological processes. Herein, we…”
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Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery by Bertschinger, Martin, Backliwal, Gaurav, Schertenleib, Arnaud, Jordan, Martin, Hacker, David L., Wurm, Florian M.

“…Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various…”
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High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens by Counoupas, Claudio, Pino, Paco, Stella, Alberto O, Ashley, Caroline, Lukeman, Hannah, Bhattacharyya, Nayan D, Tada, Takuya, Anchisi, Stephanie, Metayer, Charles, Martinis, Jacopo, Aggarwal, Anupriya, Dcosta, Belinda M, Britton, Warwick J, Kint, Joeri, Wurm, Maria J, Landau, Nathaniel R, Steain, Megan, Turville, Stuart G, Wurm, Florian M, David, Sunil A, Triccas, James A

“…Global control of COVID-19 will require the deployment of vaccines capable of inducing long-term protective immunity against SARS-CoV-2 variants. In this…”
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Serum-free large-scale transient transfection of CHO cells by Derouazi, Madiha, Girard, Philippe, Van Tilborgh, Frédéric, Iglesias, Keyvan, Muller, Natalie, Bertschinger, Martin, Wurm, Florian M.

“…To date, methods for large‐scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine…”
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Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells by Wulhfard, Sarah, Tissot, Stéphanie, Bouchet, Sophie, Cevey, Jean, de Jesus, Maria, Hacker, David L., Wurm, Florian M.

“…Large‐scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted…”
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