Cytokine release by ovine macrophages following infection with Chlamydia psittaci

Chlamydia psittaci is an obligate intracellular pathogen that causes abortion in both sheep and humans. The disease in sheep (but not humans) is characterized by a long‐term persistent phase that appears to be under the control of interferon‐gamma. However, nothing is known about cytokine induction...

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Published in:	Clinical and experimental immunology Vol. 117; no. 2; pp. 309 - 315
Main Authors:	ENTRICAN, G, WILKIE, R, MCWATERS, P, SCHEERLINCK, J.-P, WOODT, P. R, BROWN, J
Format:	Journal Article
Language:	English
Published:	Oxford BSL Blackwell Science Ltd 01-08-1999 Blackwell Oxford University Press Blackwell Science Inc
Subjects:	Animals Bacterial diseases Bacterial diseases of the respiratory system Biological and medical sciences Bronchoalveolar Lavage Fluid - cytology Bronchoalveolar Lavage Fluid - immunology Cells, Cultured Chlamydia psittaci Chlamydophila psittaci - immunology cytokines Cytokines - metabolism Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Human bacterial diseases Immunophenotyping - veterinary Infectious diseases innate immunity Interleukin-1 - metabolism Interleukin-8 - metabolism Macrophages, Alveolar - immunology Macrophages, Alveolar - metabolism Macrophages, Alveolar - microbiology Medical sciences Original ovine Psittacosis - immunology Psittacosis - pathology Psittacosis - veterinary Sheep Sheep Diseases - immunology Sheep Diseases - pathology Animal model Chlamydiaceae Cytokine Chlamydia psittaci Natural immunity Interleukin 1β Infection Vertebrata Mammalia Pulmonary alveolus Animal Chlamydiales Bacteriosis Bacteria Sheep Artiodactyla Ungulata Granulocyte macrophage colony stimulating factor Interleukin 8 Macrophage
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Summary:	Chlamydia psittaci is an obligate intracellular pathogen that causes abortion in both sheep and humans. The disease in sheep (but not humans) is characterized by a long‐term persistent phase that appears to be under the control of interferon‐gamma. However, nothing is known about cytokine induction that precedes the persistent phase in sheep. Primary alveolar lavage cells recovered from normal adult sheep were used to study cytokine production in the first 72 h of infection with C. psittaci. These cells were phenotypically characteristic of macrophages, being adherent, phagocytic, CD14+ and staining positive for non‐specific esterase. In vitro infection of the macrophages with C. psittaci resulted in the release of IL‐1β, IL‐8 and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) as measured by ovine‐specific ELISAs. Heat‐treated chlamydiae (1 h at 65°C) did not induce the release of IL‐1β, but the release of IL‐8 was similar to that induced by untreated organisms. The cells from different sheep varied most notably in their patterns of GM‐CSF release in response to heat‐treated and untreated organisms.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0009-9104 1365-2249
DOI:	10.1046/j.1365-2249.1999.00972.x