Search Results - "WEEDS, Alan G"

Modulation of actin filament dynamics by actin-binding proteins residing in lamellipodia by Mazur, Antonina Joanna, Gremm, Dagmar, Dansranjavin, Temuujin, Litwin, Monika, Jockusch, Brigitte M., Wegner, Albrecht, Weeds, Alan G., Mannherz, Hans Georg

“…Lamellipodial extension depends essentially on the polymerisation cycle of actin. In this cellular compartment the rate and extent of actin polymerisation is…”
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Mapping the ADF/Cofilin Binding Site on Monomeric Actin by Competitive Cross-linking and Peptide Array: Evidence for a Second Binding Site on Monomeric Actin by Mannherz, Hans Georg, Ballweber, Edda, Galla, Marco, Villard, Sylvie, Granier, Claude, Steegborn, Clemens, Schmidtmann, Anja, Jaquet, Kornelia, Pope, Brian, Weeds, Alan G.

“…The binding sites for actin depolymerising factor (ADF) and cofilin on G-actin have been mapped by competitive chemical cross-linking using deoxyribonuclease I…”
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Uncoupling actin filament fragmentation by cofilin from increased subunit turnover by Pope, Brian J, Gonsior, Sabine M, Yeoh, Sharon, McGough, Amy, Weeds, Alan G

“…The actin depolymerizing factor (ADF)/cofilin family of proteins interact with actin monomers and filaments in a pH-sensitive manner. When ADF/cofilin binds…”
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Gelsolin Domains 4–6 in Active, Actin-free Conformation Identifies Sites of Regulatory Calcium Ions by Kolappan, Subramaniapillai, Gooch, John T., Weeds, Alan G., McLaughlin, Paul J.

“…Structural analysis of gelsolin domains 4–6 demonstrates that the two highest-affinity calcium ions that activate the molecule are in domains 5 and 6, one in…”
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Thymosin beta4 inhibits ADF/cofilin stimulated F-actin cycling and hela cell migration: Reversal by active Arp2/3 complex by Al Haj, Abdulatif, Mazur, Antonina Joanna, Buchmeier, Sabine, App, Christine, Theiss, Carsten, Silvan, Unai, Schoenenberger, Cora-Ann, Jockusch, Brigitte M., Hannappel, Ewald, Weeds, Alan G., Mannherz, Hans Georg

“…F‐actin treadmilling plays a key part in cell locomotion. Because immunofluorescence showed colocalisation of thymosin beta4 (Tβ4) with cofilin‐1 and Arp2/3…”
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Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor by Pope, Brian J, Zierler-Gould, Karen M, Kühne, Ronald, Weeds, Alan G, Ball, Linda J

“…Human actin-depolymerizing factor (ADF) and cofilin are pH-sensitive, actin-depolymerizing proteins. Although 72% identical in sequence, ADF has a much higher…”
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F-Actin and G-Actin Binding are Uncoupled by Mutation of Conserved Tyrosine Residues in Maize Actin Depolymerizing Factor (ZmADF) by Jiang, Chang-Jie, Weeds, Alan G., Khan, Safina, Hussey, Patrick J.

“…Actin depolymerizing factors (ADF) are stimulus responsive actin cytoskeleton modulating proteins. They bind both monomeric actin (G-actin) and filamentous…”
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Regulation of the pollen-specific actin-depolymerizing factor LlADF1 by Allwood, Ellen G, Anthony, Richard G, Smertenko, Andrei P, Reichelt, Stefanie, Drobak, Bjorn K, Doonan, John H, Weeds, Alan G, Hussey, Patrick J

“…Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the…”
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The two ADF-H domains of twinfilin play functionally distinct roles in interactions with actin monomers by Ojala, Pauli J, Paavilainen, Ville O, Vartiainen, Maria K, Tuma, Roman, Weeds, Alan G, Lappalainen, Pekka

“…Twinfilin is a ubiquitous and abundant actin monomer-binding protein that is composed of two ADF-H domains. To elucidate the role of twinfilin in actin…”
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Dual effects of staurosporine on A431 and NRK cells: Microfilament disassembly and uncoordinated lamellipodial activity followed by cell death by Mannherz, Hans G., Gonsior, Sabine M., Wu, Xueqing, Polzar, Bernhard, Pope, Brian J., Wartosch, Lena, Weeds, Alan G.

“…The general protein kinase inhibitor staurosporine (STS) has dual effects on human epidermoid cancer cells (A431) and normal rat kidney fibroblasts (NRK). It…”
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Activated cofilin colocalises with Arp2/3 complex in apoptotic blebs during programmed cell death by Mannherz, Hans G., Gonsior, Sabine M., Gremm, Dagmar, Wu, Xueqing, Pope, Brian J., Weeds, Alan G.

“…Etoposide inhibits topoisomerase II and induces apoptosis in human epidermoid cancer cells (A431) and normal rat fibroblasts (NRK) as verified by apoptotic…”
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Ser6 in the maize actin‐depolymerizing factor, ZmADF3, is phosphorylated by a calcium‐stimulated protein kinase and is essential for the control of functional activity by Smertenko, Andrei P., Jiang, Chang‐Jie, Simmons, Nicola J., Weeds, Alan G., Davies, Dewi R., Hussey, Patrick J.

“…Summary Maize actin‐depolymerizing factor, ZmADF, binds both G‐ and F‐actin and enhances in vitro actin dynamics. Evidence from studies on vertebrate…”
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The C-terminal Tail of UNC-60B (Actin Depolymerizing Factor/Cofilin) Is Critical for Maintaining Its Stable Association with F-actin and Is Implicated in the Second Actin-binding Site by Ono, S, McGough, A, Pope, B J, Tolbert, V T, Bui, A, Pohl, J, Benian, G M, Gernert, K M, Weeds, A G

“…Actin depolymerizing factor (ADF)/cofilin changes the twist of actin filaments by binding two longitudinally associated actin subunits. In the absence of an…”
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Equilibria and Kinetics of Folding of Gelsolin Domain 2 and Mutants Involved in Familial Amyloidosis-Finnish Type by Isaacson, R L, Weeds, A G, Fersht, A R

“…Mutations D187N and D187Y in domain 2 of the actin-regulating protein gelsolin cause familial amyloidosis-Finnish type (FAF). We have constructed and expressed…”
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Interaction of pollen-specific actin-depolymerizing factor with actin by SMERTENKO, Andrei P, ALLWOOD, Ellen G, KHAN, Safina, JIANG, Chang-Jie, MACIVER, Sutherland K, WEEDS, Alan G, HUSSEY, Patrick J

“…We have examined the interaction of recombinant lily pollen ADF, LlADF1, with actin and found that whilst it bound both G- and F-actin, it had a much smaller…”
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Actophorin preferentially binds monomeric ADP-Actin over ATP-bound actin: consequences for cell locomotion by Maciver, Sutherland K., Weeds, Alan G.

“…Actophorin from Acanthamoeba castellanii severs actin filaments and sequesters actin monomers. Here we report that actophorin binds ADP-bound monomers with…”
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Probing the Effects of Calcium on Gelsolin by Pope, Brian J, Gooch, John T, Weeds, Alan G

“…Gelsolin is a calcium-regulated actin severing and capping protein that binds two calcium ions and has three sites for actin; two recognize monomeric actin and…”
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Solution Structure of Human Cofilin by Pope, Brian J., Zierler-Gould, Karen M., Kühne, Ronald, Weeds, Alan G., Ball, Linda J.

“…Human actin-depolymerizing factor (ADF) and cofilin are pH-sensitive, actin-depolymerizing proteins. Although 72% identical in sequence, ADF has a much higher…”
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Studies on the chymotryptic digestion of myosin. Effects of divalent cations on proteolytic susceptibility by Weeds, A G, Pope, B

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Regulation of the Pollen-Specific Actin-Depolymerizing Factor LIADF1 by Allwood, Ellen G., Anthony, Richard G., Smertenko, Andrei P., Reichelt, Stefanie, Drobak, Bjorn K., Doonan, John H., Weeds, Alan G., Hussey, Patrick J.

“…Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the…”
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