Rat lung contains a developmentally regulated manganese superoxide dismutase mRNA-binding protein

Rat lung contains a developmentally regulated manganese superoxide dismutase mRNA-binding protein

It has become increasingly clear that RNA-binding proteins play an important role in the regulation of gene expression. The presence in rat lung of a specific, redox-sensitive catalase RNA-binding protein was recently reported (Clerch, L. B., and D. Massaro, 1992. J. Biol. Chem. 267:2853). In order...

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Bibliographic Details
Published in:The Journal of clinical investigation Vol. 92; no. 3; pp. 1278 - 1281
Main Authors: FAZZONE, H, WANGNER, A, CIERCH, L. B
Format: Journal Article
Language:English
Published: Ann Arbor, MI American Society for Clinical Investigation 01-09-1993
Subjects:
Animals
Binding Sites
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene expression
Lung - embryology
Lung - metabolism
Molecular and cellular biology
Molecular genetics
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
RNA-Binding Proteins - metabolism
Superoxide Dismutase - genetics
RNA
Rat
Enzyme
Lung
Rodentia
Superoxide dismutase
Gene expression
Probe
Binding protein
Vertebrata
Mammalia
Animal
Physiology
Age
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Summary:It has become increasingly clear that RNA-binding proteins play an important role in the regulation of gene expression. The presence in rat lung of a specific, redox-sensitive catalase RNA-binding protein was recently reported (Clerch, L. B., and D. Massaro, 1992. J. Biol. Chem. 267:2853). In order to determine if specific manganese superoxide dismutase (MnSOD) RNA-binding proteins exist, we tested whether protein in rat lung extract would bind to 32P-labeled MnSOD RNA. Using a gel mobility shift assay we show rat lung protein forms specific complexes with a 216 b fragment of the 3' untranslated region of MnSOD RNA and the binding requires the presence of free sulfhydryl groups. Competition studies indicate MnSOD RNA-binding protein is different from catalase RNA-binding protein. Furthermore, unlike catalase RNA-binding protein, rat lung MnSOD RNA-binding protein activity is developmentally regulated; there is less MnSOD RNA-protein binding activity in adult rat lung extract compared to prenatal or neonatal rat lung extracts. We conclude the lung contains developmentally regulated MnSOD mRNA-binding protein that is redox sensitive.
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ISSN:0021-9738
1558-8238
DOI:10.1172/jci116700