Search Results - "Wünsche, Winfried"

Cell stress is related to re-localization of Argonaute 2 and to decreased RNA interference in human cells by Detzer, Anke, Engel, Christina, Wünsche, Winfried, Sczakiel, Georg

“…Various kinds of stress on human cells induce the formation of endogenous stress granules (SGs). Human Argonaute 2 (hAgo2), the catalytic core component of the…”
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Increased RNAi is related to intracellular release of siRNA via a covalently attached signal peptide by Detzer, Anke, Overhoff, Marita, Wünsche, Winfried, Rompf, Maria, Turner, John J, Ivanova, Gabriela D, Gait, Michael J, Sczakiel, Georg

“…In the last decade short interfering RNA (siRNA) became an important means for functional genomics and the development of gene-specific drugs. However, major…”
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Quantitative detection of siRNA and single-stranded oligonucleotides: relationship between uptake and biological activity of siRNA by Overhoff, Marita, Wünsche, Winfried, Sczakiel, Georg

“…The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we…”
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Cellular uptake and intracellular release are major obstacles to the therapeutic application of siRNA: novel options by phosphorothioate-stimulated delivery by Mescalchin, Alessandra, Detzer, Anke, Wecke, Maria, Overhoff, Marita, Wünsche, Winfried, Sczakiel, Georg

“…The cellular uptake of oligomeric nucleic acid-based tools and drugs including small-interfering RNA (siRNA) represents a major technical hurdle for the…”
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Tamoxifen exerts agonistic effects on clusterin and complement C3 gene expression in RUCA-I primary xenografts and metastases but not normal uterus by Zierau, Oliver, O’Sullivan, Jacintha, Morrissey, Colm, McDonald, Dana, Wünsche, Winfried, Schneider, Martin R, Tenniswood, Martin P, Vollmer, Günter

“…Tamoxifen is the most widely prescribed anti-neoplastic drug for the treatment of both localized and metastatic breast cancer. It is also the prototype for a…”
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Specific Recognition of Proteins by Array-Bound Hexanucleotides by Mescalchin, Alessandra, Wünsche, Winfried, Sczakiel, Georg

“…Maximum information density: The complete hexanucleotide sequence space can be immobilized on a chip. The binding of a number of proteins was profiled with…”
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Concentrations of Circulating RNA from Healthy Donors and Cancer Patients Estimated by Different Methods by RYKOVA, ELENA Y., WUNSCHE, WINFRIED, BRIZGUNOVA, OLGA E., SKVORTSOVA, TATYANA E., TAMKOVICH, SVETLANA N., SENIN, ILIJA S., LAKTIONOV, PAVEL P., SCZAKIEL, GEORG, VLASSOV, VALENTIN V.

“…:  Circulating RNA (cirRNA) was isolated from plasma and cell surface‐bound fractions of blood of healthy women and breast cancer patients. RNA samples were…”
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Functional binding of hexanucleotides to 3C protease of hepatitis A virus by Blaum, Bärbel S, Wünsche, Winfried, Benie, Andrew J, Kusov, Yuri, Peters, Hannelore, Gauss-Müller, Verena, Peters, Thomas, Sczakiel, Georg

“…Oligonucleotides as short as 6 nt in length have been shown to bind specifically and tightly to proteins and affect their biological function. Yet, sparse…”
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Characterization of Estrogenicity of Phytoestrogens in an Endometrial-Derived Experimental Model by Hopert, Anne-Christine, Beyer, Antje, Frank, Kirsten, Strunck, Elisabeth, Wünsche, Winfried, Vollmer, Günter

“…Severe developmental and reproductive disorders in wild animals have been linked to high exposure to persistent environmental chemicals with hormonal activity…”
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The Activity of siRNA in Mammalian Cells is Related to the Kinetics of siRNA-target Recognition In Vitro: Mechanistic Implications by Wünsche, Winfried, Sczakiel, Georg

“…The specificity of siRNA-mediated suppression of gene expression involves base–base recognition between siRNA and its single-stranded RNA target. We…”
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Spezifische Proteinerkennung durch Array-gebundene Hexanukleotide by Mescalchin, Alessandra, Wünsche, Winfried, Sczakiel, Georg

“…Ein Chip bietet Platz für den gesamten Hexanukleotid‐Sequenzraum in Form eines Arrays. Unter den Proteinen, deren Bindungsverhalten bezüglich dieser 4096…”
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Specific binding of a hexanucleotide to HIV-1 reverse transcriptase: a novel class of bioactive molecules by Mescalchin, Alessandra, Wünsche, Winfried, Laufer, Sandra D, Grohmann, Dina, Restle, Tobias, Sczakiel, Georg

“…Short oligonucleotides below 8-10 nt in length adopt relatively simple structures. Accordingly, they represent interesting and so far unexplored lead compounds…”
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hexanucleotide selected for increased cellular uptake in cis contains a highly active CpG-motif in human B cells and primary peripheral blood mononuclear cells by Mende, Miriam, Hopert, Anne, Wünsche, Winfried, Overhoff, Marita, Detzer, Anke, Börngen, Kirsten, Schlenke, Peter, Kirchner, Holger, Sczakiel, Georg

“…The relationship between immunostimulation of human B cells by cytosine-phosphate-guanosine (CpG) -containing oligonucleotides and their physical cellular…”
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Estrogenic and antiestrogenic regulation of MMP-2 and MMP-13 mRNA in RUCA-I endometrial tumor cells in vitro and in vivo by Tüshaus, Ludger, Hopert, Anne-Christine, Strunck, Elisabeth, Schubert, Christine, Wünsche, Winfried, Vollmer, Günter

“…We investigated the influence of estrogenic and antiestrogenic treatment on proteolytic activity – especially on MMP-2 and MMP-13 – in the RUCA-I…”
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Relative binding affinity does not predict biological response to xenoestrogens in rat endometrial adenocarcinoma cells by Strunck, Elisabeth, Stemmann, Nicole, Hopert, Anne-Christine, Wünsche, Winfried, Frank, Kirsten, Vollmer, Günter

“…The possible adverse effects of the so-called environmental estrogens have raised considerable concern. Developmental, endocrine and reproductive disorders in…”
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Estrogenic regulation of clusterin mRNA in normal and malignant endometrial tissue by Wünsche, Winfried, Tenniswood, Martin P., Schneider, Martin R., Vollmer, Günter

“…Clusterin is a heterodimeric, 80kDa, glycoprotein that is synthesized in a wide variety of tissues in response to a number of diverse stimuli, including…”
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Extracellular matrix induces hormone responsiveness and differentiation in RUCA-I rat endometrial adenocarcinoma cells by Vollmer, Günter, Ellerbrake, Nicola, Hopert, Anne-Christine, Knauthe, Rudolf, Wünsche, Winfried, Knuppen, Rudolf

“…We recently described the establishment and the characterization of two rat endometrial adenocarcinoma cell lines which we called RUCA-I and RUCA-II. Despite…”
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Estrogen-dependent and cell-specific regulation of gene expression in RUCA-I endometrial adenocarcinoma cells by Hopert, Anne-Christine, Beyer, Antje, Strunck, Elisabeth, Wünsche, Winfried, Vollmer, Günter

“…Estrogens are believed to play a crucial role in growth regulation and differentiation of the normal endometrial tissue as well as in the carcinogenesis of the…”
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Fibronectin is an estrogen-repressed protein in RUCA-I rat endometrial adenocarcinoma cells by Vollmer, Günter, Hopert, Anne-Christine, Ellerbrake, Nicola, Wünsche, Winfried, Knuppen, Rudolf

“…We recently established and characterized two rat endometrial adenocarcinoma cell lines which we called RUCA-I and RUCA-II. Despite high estrogen receptor…”
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Methyl and bromo derivatives of estradiol are agonistic ligands for the estrogen receptor of MCF-7 breast cancer cells by Vollmer, G, Wünsche, W, Schütze, N, Feit, B, Knuppen, R

“…The binding affinity and relative estrogenic potency of 2-bromo-, 4-bromo-, 2-methyl- and 4-methylestradiol was evaluated in MCF-7 breast cancer cells. The…”
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