Uncoupling effect of lauryl sulfate on mitochondria can be mediated by release of bound endogenous fatty acids

The mechanism of uncoupling by lauryl sulfate (LS) has been studied. The very fact that uncoupling by low concentration of LS (a strong acid) resembles very much that by fatty acids (weak acids) was used as an argument against the fatty acid cycling scheme of uncoupling where protonated fatty acids...

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Bibliographic Details
Published in:	Biochemistry (Moscow) Vol. 71; no. 12; pp. 1365 - 1369
Main Authors:	Simonian, R A, Pustovidko, A V, Vyssokikh, M Iu, Skulachev, V P
Format:	Journal Article
Language:	English
Published:	United States Springer Nature B.V 01-12-2006
Subjects:	Adenosine diphosphate Adenosine Diphosphate - chemistry Adenosine Diphosphate - metabolism Adenosine Triphosphate - chemistry Adenosine Triphosphate - metabolism Animals Antiporters - antagonists & inhibitors Antiporters - chemistry Antiporters - metabolism Atractyloside - analogs & derivatives Atractyloside - chemistry Atractyloside - pharmacology Fatty acids Fatty Acids - chemistry Fatty Acids - metabolism Mitochondria Mitochondria, Liver - chemistry Mitochondria, Liver - metabolism Mitochondrial Membranes - chemistry Mitochondrial Membranes - metabolism Proteins Rats Sodium Dodecyl Sulfate - chemistry Sodium Dodecyl Sulfate - pharmacology Sulfates Surface-Active Agents - chemistry Surface-Active Agents - pharmacology Uncoupling Agents - chemistry Uncoupling Agents - pharmacology
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Summary:	The mechanism of uncoupling by lauryl sulfate (LS) has been studied. The very fact that uncoupling by low concentration of LS (a strong acid) resembles very much that by fatty acids (weak acids) was used as an argument against the fatty acid cycling scheme of uncoupling where protonated fatty acids operate as a protonophore. We have found that rat liver and heart muscle mitochondria can be uncoupled by low (70 microM) LS concentration in a fashion completely arrested by the ATP/ADP antiporter inhibitor carboxyatractylate (CAtr). On the other hand, uncoupling by two-fold higher LS concentration is not sensitive to CAtr. Addition of oleate desensitizes mitochondria to low LS so that addition of bovine serum albumin becomes necessary to recouple mitochondria. The data are accounted for assuming that low LS releases endogenous fatty acids from some mitochondrial depots, and these fatty acids are responsible for uncoupling. As to high LS, it causes a nonspecific (CAtr-insensitive) damage to the mitochondrial membrane.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-2979 1608-3040
DOI:	10.1134/S0006297906120121