Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

We compared the formation of γ H2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3 H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linea...

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Bibliographic Details
Published in:	Bulletin of experimental biology and medicine Vol. 172; no. 2; pp. 245 - 249
Main Authors:	Rodneva, S. M., Osipov, A. A., Guryev, D. V., Tsishnatti, A. A., Fedotov, Y. А., Yashkina, E. I., Vorobyova, N. Y., Maksimov, A. A., Kochetkov, O. A., Samoylov, A. S., Osipov, A. N.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-12-2021 Springer Nature B.V
Subjects:	Alanine Amino acids Amino Acids - chemistry Amino Acids - pharmacology Biomedical and Life Sciences Biomedicine Cell Biology Cells, Cultured Chromatin Comparative analysis Culture Media - chemistry Culture Media - pharmacology Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded - radiation effects DNA damage Fibroblasts Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Fibroblasts - radiation effects Histones - genetics Histones - metabolism Humans Internal Medicine Laboratory Medicine Lung - drug effects Lung - metabolism Lung - pathology Lung - radiation effects Neoplastic Stem Cells - drug effects Neoplastic Stem Cells - radiation effects Pathology Proline Radioactivity Thymidine Thymidine - chemistry Thymidine - pharmacology Tritium Tritium - chemistry Tritium - pharmacology H-thymidine H-amino acids tritium MRC-5 γH2AX 3H-thymidine 3H-amino acids
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Summary:	We compared the formation of γ H2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3 H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γ H2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3 H-thymidine was more than 2-fold higher than under the influence of 3 H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3 H-labeled amino acids showed that 3 H-alanine produced more pronounced effect that 3 H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-021-05370-6