Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging

Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibil...

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Bibliographic Details
Published in:	Experimental eye research Vol. 203; p. 108394
Main Authors:	Tkachev, Sergey Y., Mitrin, Boris I., Karnaukhov, Nikolay S., Sadyrin, Evgeniy V., Voloshin, Mark V., Maksimov, Alexey Y., Goncharova, Anna S., Lukbanova, Ekaterina A., Zaikina, Ekaterina V., Volkova, Anastasiya V., Khodakova, Darya V., Mindar, Maria V., Yengibarian, Marina A., Protasova, Tatyana P., Kit, Sergey O., Ermakov, Alexey M., Chapek, Sergey V., Tkacheva, Marina S.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-02-2021
Subjects:	Anterior Chamber - anatomy & histology Anterior Chamber - diagnostic imaging Anterior Eye Segment - anatomy & histology Anterior Eye Segment - diagnostic imaging Eye - anatomy & histology Eye - diagnostic imaging Eye Enucleation Feasibility Studies Humans Imaging, Three-Dimensional Lens, Crystalline - anatomy & histology Lens, Crystalline - diagnostic imaging Retina - anatomy & histology Retina - diagnostic imaging X-Ray Microtomography
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Summary:	Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol. We also identified the challenges of applying this contrasting technique to lens, cornea, and retina and proposed alternative staining methods for these tissues. Thereby this work provides a starting point for other studies for imaging of human eyes in normal and pathological conditions using lab-based micro-CT systems. •Application of laboratory-based micro-CT imaging for the investigation of the human eye.•Advantages and disadvantages of iodine in ethanol (I2E) staining of the human eye structures for the further micro-CT imaging.•Identification of clinically important structures of the human eye based on 2D sections and 3D volume-rendering models.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4835 1096-0007
DOI:	10.1016/j.exer.2020.108394