Search Results - "Volke, Daniel C"
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Industrial biotechnology of Pseudomonas putida: advances and prospects
Published in Applied microbiology and biotechnology (01-09-2020)“…Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in diverse ecological habitats. This ubiquity is traced to its remarkably…”
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A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida
Published in Nature communications (07-10-2020)“…Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures…”
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Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
Published in Nature communications (31-05-2022)“…CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use in non-traditional bacteria depends on host factors or exogenous…”
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Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida
Published in Microbial biotechnology (01-01-2020)“…Summary Most of the gene expression systems available for Gram‐negative bacteria are afflicted by relatively high levels of basal (i.e. leaky) expression of…”
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SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
Published in Nucleic acids research (06-01-2023)“…The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic…”
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A versatile microbial platform as a tunable whole-cell chemical sensor
Published in Nature communications (27-09-2024)“…Biosensors are used to detect and quantify chemicals produced in industrial microbiology with high specificity, sensitivity, and portability. Most biosensors,…”
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Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida
Published in Metabolic engineering communications (01-06-2020)“…Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative,…”
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An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida
Published in Microbial biotechnology (01-03-2020)“…Summary Owing to its wide metabolic versatility and physiological robustness, together with amenability to genetic manipulations and high resistance to…”
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Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator
Published in Microbial biotechnology (01-07-2019)“…Summary The ability of Pseudomonas species to thrive in all major natural environments (i.e. terrestrial, freshwater and marine) is based on its exceptional…”
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Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production
Published in Microbial biotechnology (01-03-2024)“…Paenibacillus polymyxa is a non‐pathogenic, Gram‐positive bacterium endowed with a rich and versatile metabolism. However interesting, this bacterium has been…”
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Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors
Published in Engineering in life sciences (01-03-2021)“…In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was…”
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Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
Published in Bio-protocol (20-02-2021)“…Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional…”
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13
Pseudomonas putida
Published in Trends in microbiology (Regular ed.) (01-06-2020)Get full text
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Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Published in Current opinion in microbiology (01-10-2023)“…Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated proteins (Cas) technologies brought a transformative change in the way…”
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Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes
Published in Trends in biotechnology (Regular ed.) (01-10-2022)“…Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple chemicals, yet successful industrial processes are relatively…”
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Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Published in New biotechnology (25-05-2023)“…Automation is playing an increasingly significant role in synthetic biology. Groundbreaking technologies, developed over the past 20 years, have enormously…”
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Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana
Published in Proceedings of the National Academy of Sciences - PNAS (09-03-2021)“…Insect damage to plants is known to up-regulate defense and down-regulate growth processes. While there are frequent reports about up-regulation of defense…”
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Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida
Published in Metabolic engineering (01-09-2023)“…Pseudomonas putida, a microbial host widely adopted for metabolic engineering, processes glucose through convergent peripheral pathways that ultimately yield…”
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A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis
Published in ACS catalysis (03-06-2022)“…Fluorinases, the only enzymes known to catalyze the transfer of fluorine to an organic molecule, are essential catalysts for the biological synthesis of…”
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Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded metabolic model
Published in Metabolic engineering (01-01-2023)“…Pseudomonas putida KT2440 is an attractive bacterial host for biotechnological production of valuable chemicals from renewable lignocellulosic feedstocks as it…”
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