Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato

The sequence variants of the signal recognition particle (SRP) RNA gene family from four tomato cultivars have been isolated and characterized which indicated the existence of SRP RNA pseudogenes. Sequence analysis revealed two conserved sequence motifs in the upstream region, a TATA-like box and an...

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Bibliographic Details
Published in:	Plant molecular biology Vol. 31; no. 1; pp. 113 - 125
Main Authors:	Riedel, L. (Max-Planck-Institut fuer Biochemie, Martinsried (Germany). Abteilung Viroidforschung), Volger, U, Luckinger, R, Puetz, A, Saenger, H.L, Wassenegger, M
Format:	Journal Article
Language:	English
Published:	Netherlands 01-04-1996
Subjects:	ARN Base Sequence Cloning, Molecular Conserved Sequence GENE Gene Expression Regulation, Plant GENES LYCOPERSICON ESCULENTUM Lycopersicon esculentum - genetics Molecular Sequence Data Multigene Family NUCLEOPROTEINAS NUCLEOPROTEINE NUCLEOPROTEINS NUCLEOTIDE SEQUENCE Plant Leaves - genetics Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Restriction Mapping RNA RNA, Plant - genetics SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE Signal Recognition Particle - genetics
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Summary:	The sequence variants of the signal recognition particle (SRP) RNA gene family from four tomato cultivars have been isolated and characterized which indicated the existence of SRP RNA pseudogenes. Sequence analysis revealed two conserved sequence motifs in the upstream region, a TATA-like box and an upstream sequence element (USE), 'TCCCACATCG', both located at a conserved distance to the transcription start point. These elements are identical to the DNA-dependent RNA polymerase III (pol III)-specific promoters of U-rich small nuclear RNA (UsnRNA) genes of plants. Moreover, T-rich stretches are found at the 3' end of the coding regions of the SRP RNA genes which could act as typical pol III termination signals. These findings and recent results from site-directed mutation analysis of the SRP RNA genes from Arabidopsis thaliana indicate that, in contrast to mammalian systems, plant pol III SRP RNA genes are most probably regulated by external promoter elements. According to the identical promoter organization between plant U3-, U6snRNA, MRP-like RNA and SRP RNA genes, one can group these genes into the 'pol III(EXT)USE' subclass of externally regulated USE-dependent pol III genes.
Bibliography:	F30 9700642 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0167-4412 1573-5028
DOI:	10.1007/BF00020611