Plasma high sensitivity C-reactive protein and its relationship with cytokine levels in children with newly diagnosed type 1 diabetes and ketoacidosis

High-sensitivity C-reactive protein (hs-CRP) and pro-inflammatory cytokines have been suggested as sensitive markers of endothelial dysfunction. Our aim was to monitor plasma hs-CRP levels at different time-points and in different degrees of ketoacidosis severity, its association with cytokine level...

Full description

Saved in:

Bibliographic Details
Published in:	Clinical biochemistry Vol. 45; no. 16-17; pp. 1383 - 1388
Main Authors:	Karavanaki, Kyriaki, Kakleas, Kostas, Georga, Soultana, Bartzeliotou, Αnastasia, Mavropoulos, George, Tsouvalas, Manolis, Vogiatzi, Alice, Papassotiriou, Ioannis, Karayianni, Christina
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-11-2012 Elsevier
Subjects:	Analysis of Variance Biological and medical sciences Biomarkers - blood Brain Edema - blood Brain Edema - etiology C-Reactive Protein - metabolism Capillary perturbation Child Child, Preschool Children Cytokines - blood Diabetes Mellitus, Type 1 - blood Diabetes Mellitus, Type 1 - complications Diabetes Mellitus, Type 1 - diagnosis Diabetes. Impaired glucose tolerance Diabetic ketoacidosis Diabetic Ketoacidosis - blood Diabetic Ketoacidosis - diagnosis Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Female hs-CRP Humans IL-6 Leukocyte Count Male Medical sciences Pulmonary Edema - blood Pulmonary Edema - etiology Severe DKA complications Statistics, Nonparametric Severe DKA complications Children Capillary perturbation hs-CRP IL-6 Diabetic ketoacidosis Endocrinopathy Human Immunopathology Endothelial cell Pathophysiology Cytokine Autoimmune disease Children hs-CRP Acid base balance disorder Interleukin 6 Metabolic disorder Acute phase protein Type 1 diabetes Immunological investigation Dysfunction Ketoacidosis Clinical biology Complication C reactive protein Child
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	High-sensitivity C-reactive protein (hs-CRP) and pro-inflammatory cytokines have been suggested as sensitive markers of endothelial dysfunction. Our aim was to monitor plasma hs-CRP levels at different time-points and in different degrees of ketoacidosis severity, its association with cytokine levels and its role as a marker of severe ketoacidosis complications. We studied in 38 newly diagnosed children with type 1 diabetes and ketoacidosis, aged 7.7±3.1years, hs-CRP, white blood cell count (WBC), and plasma levels of cytokines IL-1β (interleukin-1β), IL-2, IL-6, IL-8, IL-10, TNF-α (tumor necrosis factor-α) prior to and during DKA management. On admission, the levels of WBC, PMN, IL-6 and IL-10 were elevated, but were all reduced within 120h after ketoacidosis management. In the group with moderate/severe ketoacidosis, but not in mild ketoacidosis, hs-CRP levels were significantly reduced at 24h (p=0.021), WBC and IL-6 at 120h (p=0.003), while IL-10 was prematurely reduced at 6–8h (p=0.008). Moreover hs-CRP was significantly associated with WBC (p=0.023) and IL-6 (p=0.028) on admission, with IL-6 (p=0.002) and IL-8 (p=0.014) at 24h and with IL-10 (p=0.027) at 120h. The above were not observed in the group with mild ketoacidosis. In the children with moderate/severe diabetic ketoacidosis of our study, increased levels of hs-CRP and IL-6 were observed, together with leukocytosis and neutrophilia, without the presence of infection. As hs-CRP was found to be strongly associated with the inflammatory IL-6, the prolonged elevation of hs-CRP levels in children with severe ketoacidosis could serve as a marker for the development of its severe complications. ► Increased levels of hs-CRP and IL-6 were associated with leukocytosis and neutrophilia. ► hs-CRP strongly associated with IL-6. ► Prolonged elevation of hs-CRP could serve as a marker for the development of severe complications.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0009-9120 1873-2933
DOI:	10.1016/j.clinbiochem.2012.05.003