Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera)

The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (<Glu-Leu-Thr-Phe-Thr-Pro-Gly-Tr...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 86; no. 20; pp. 8161 - 8164
Main Authors:	Jaffe, H. (ARS, USDA, Beltsville, MD), Raina, A.K, Riley, C.T, Fraser, B.A, Nachman, R.J, Vogel, V.W, Zhang, Y.S, Hayes, D.K
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences of the United States of America 01-10-1989 National Acad Sciences
Subjects:	Agriculture Amino Acid Sequence Amino acids Animals Corpora cardiaca DIPTERA Diptera - drug effects Diptera - physiology Dose response relationship Enzymes Flies Hemolymph Hormones Horses hypotrehalosemic hormone Insect Hormones - chemical synthesis Insect Hormones - pharmacology Lipids Male Mass Spectrometry Molecular Sequence Data Musca autumnalis Muscidae Nervous System Physiological Phenomena Neuropeptides Oligopeptides - chemical synthesis Oligopeptides - pharmacology PEPTIDE PEPTIDES PEPTIDOS Pyrrolidonecarboxylic Acid - analogs & derivatives SYNTHESIS TABANIDAE TABANUS Tabanus atratus
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Summary:	The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (<Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-NH2) and Taa-HoTH (<Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-Gly-Tyr-NH2) (where <Glu = pyroglutamic acid) were determined by automated gas-phase Edman degradation of the peptides deblocked by pyroglutamate aminopeptidase and by fast atom bombardment mass spectrometry. The hormones were synthesized, and the natural and synthetic peptides exhibited identical chromatographic, spectroscopic, and biological properties. When assayed in adult face fly males, Taa-AKH and Taa-HoTH demonstrated hyperlipemic activity; in addition, Taa-HoTH also demonstrated a significant hypotrehalosemic activity.
Bibliography:	L50 9022147 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.86.20.8161