Search Results - "Vogel, Kurt W"

Parkinson disease-associated mutation R1441H in LRRK2 prolongs the "active state" of its GTPase domain by Liao, Jingling, Wu, Chun-Xiang, Burlak, Christopher, Zhang, Sheng, Sahm, Heather, Mu Wang, Zhang, Zhong-Yin, Vogel, Kurt W., Federici, Mark, Riddle, Steve M., Nichols, R. Jeremy, Liu, Dali, Cookson, Mark R., Stone, Todd A., Hoang, Quyen Q.

“…Mutation in leucine-rich-repeat kinase 2 (LRRK2) is a common cause of Parkinson disease (PD). A disease-causing point mutation R1441H/G/C in the GTPase domain…”
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Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation by Hermanson, Spencer B, Carlson, Coby B, Riddle, Steven M, Zhao, Jing, Vogel, Kurt W, Nichols, R Jeremy, Bi, Kun

“…Mutations in the leucine-rich repeat kinase-2 (LRRK2) have been linked to Parkinson's disease. Recent studies show that inhibition of LRRK2 kinase activity…”
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Pharmacological inhibition of LRRK2 cellular phosphorylation sites provides insight into LRRK2 biology by Zhao, Jing, Hermanson, Spencer B, Carlson, Coby B, Riddle, Steven M, Vogel, Kurt W, Bi, Kun, Nichols, R Jeremy

“…Mutations in LRRK2 (leucine-rich repeat kinase 2) have been linked to inherited forms of PD (Parkinson's disease). Substantial pre-clinical research and drug…”
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Multiplexing terbium- and europium-based TR-FRET readouts to increase kinase assay capacity by Horton, Robert A, Vogel, Kurt W

“…Identification and characterization of kinase inhibitor potency and selectivity is often an iterative process in which a library of compounds is first screened…”
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Threshold Levels of ABL Tyrosine Kinase Inhibitors Retained in Chronic Myeloid Leukemia Cells Determine Their Commitment to Apoptosis by O'HARE, Thomas, EIDE, Christopher A, RIDDLE, Steven M, MARKS, Bryan D, VOGEI, Kurt W, KOOP, Dennis R, APGAR, John, TYNER, Jeffrey W, DEININGER, Michael W, DRUKER, Brian J, AGARWAL, Anupriya, ADRIAN, Lauren T, ZABRISKIE, Matthew S, MACKENZIE, Ryan J, LATOCHA, Dorian H, JOHNSON, Kara J, HUIHONG YOU, LUO, Jenny

“…The imatinib paradigm in chronic myelogenous leukemia (CML) established continuous BCR-ABL inhibition as a design principle for ABL tyrosine kinase inhibitors…”
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Multiparameter analysis of a screen for progesterone receptor ligands: comparing fluorescence lifetime and fluorescence polarization measurements by Marks, Bryan D, Qadir, Naveeda, Eliason, Hildegard C, Shekhani, Mohammed Saleh, Doering, Klaus, Vogel, Kurt W

“…Direct measurement of the fluorescence lifetime (FLT) of a fluorescent label is an emerging method for high-throughput screening. Changes in the fluorescence…”
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Development and applications of a broad-coverage, TR-FRET-based kinase binding assay platform by Lebakken, Connie S, Riddle, Steven M, Singh, Upinder, Frazee, W Jack, Eliason, Hildegard C, Gao, Yi, Reichling, Laurie J, Marks, Bryan D, Vogel, Kurt W

“…The expansion of kinase assay technologies over the past decade has mirrored the growing interest in kinases as drug targets. As a result, there is no shortage…”
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Simultaneous Monitoring of Discrete Binding Events Using Dual-Acceptor Terbium-Based LRET by Kupcho, Kevin R, Stafslien, Deborah K, DeRosier, Therese, Hallis, Tina M, Ozers, Mary Szatkowski, Vogel, Kurt W

“…Lanthanide resonance energy transfer (LRET) between chelated terbium and two spectrally distinct acceptor fluorophores (fluorescein and Alexa Fluor 633) is…”
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Time-resolved fluorescence resonance energy transfer kinase assays using physiological protein substrates: Applications of terbium–fluorescein and terbium–green fluorescent protein fluorescence resonance energy transfer pairs by Riddle, Steven M., Vedvik, Kevin L., Hanson, George T., Vogel, Kurt W.

“…Fluorescence-based kinase assays using peptide substrates are an established format for high-throughput screening and profiling of kinases. Among…”
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High-throughput cellular assays for regulated posttranslational modifications by Robers, Matthew B., Horton, Robert A., Bercher, Mark R., Vogel, Kurt W., Machleidt, Thomas

“…We have developed a set of high-throughput screening (HTS)-compatible assays capable of measuring regulated, target-specific posttranslational modifications in…”
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A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein by Horton, Robert A., Strachan, Elizabeth A., Vogel, Kurt W., Riddle, Steven M.

“…Deubiquitinating enzymes (DUBs) proteolytically cleave ubiquitin from ubiquitinated proteins, and inhibition of DUBs that rescue oncogenic proteins from…”
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Development of LanthaScreen cellular assays for key components within the PI3K/AKT/mTOR pathway by Carlson, Coby B, Robers, Matthew B, Vogel, Kurt W, Machleidt, Thomas

“…The PI3K/AKT/mTOR pathway is central to cell growth and survival, cell cycle regulation, and programmed cell death. Aberrant activation of this signaling…”
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A fluorescence lifetime based binding assay to characterize kinase inhibitors by Lebakken, Connie S, Hee Chol Kang, Vogel, Kurt W

“…The authors present a fluorescence lifetime-based kinase binding assay that identifies and characterizes compounds that bind to the adenosine triphosphate…”
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A fluorescence anisotropy assay for the muscarinic M1 G-protein-coupled receptor by Huwiler, Kristin G, De Rosier, Therese, Hanson, Bonnie, Vogel, Kurt W

“…In the search for new chemical entities that interact with G-proteincoupled receptors (GPCRs), assays that quantify efficacy and affinity are employed…”
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A toolbox approach to high-throughput TR-FRET-based SUMOylation and DeSUMOylation assays by Carlson, Coby B, Horton, Robert A, Vogel, Kurt W

“…The posttranslational modification of target substrates by the ubiquitin-like proteins, specifically the small ubiquitin-like modifier (SUMO), has emerged as…”
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Development of the predictor HERG fluorescence polarization assay using a membrane protein enrichment approach by Piper, David R, Duff, Steve R, Eliason, Hildegard C, Frazee, W Jack, Frey, Elizabeth A, Fuerstenau-Sharp, Maya, Jachec, Christine, Marks, Bryan D, Pollok, Brian A, Shekhani, Mohammed Saleh, Thompson, David V, Whitney, Pam, Vogel, Kurt W, Hess, Stephen D

“…The life-threatening consequences of acquired, or drug-induced, long QT syndrome due to block of the human ether-a-go-go-related gene (hERG) channel are well…”
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Improving lanthanide-based resonance energy transfer detection by increasing donor-acceptor distances by Vogel, Kurt W, Vedvik, Kevin L

“…Lanthanide-based resonance energy transfer (LRET) is an established method for measuring or detecting proximity between a luminescent lanthanide (energy donor)…”
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Overcoming compound interference in fluorescence polarization-based kinase assays using far-red tracers by Vedvik, Kevin L, Eliason, Hildegard C, Hoffman, Randy L, Gibson, Jasmin R, Kupcho, Kevin R, Somberg, Richard L, Vogel, Kurt W

“…Kinase-mediated phosphorylation of proteins is critical to the regulation of many biological processes, including cell growth, apoptosis, and differentiation…”
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Pharmacological characterization of purified recombinant mTOR FRB-kinase domain using fluorescence-based assays by Reichling, Laurie J, Lebakken, Connie S, Riddle, Steven M, Vedvik, Kevin L, Robers, Matthew B, Kopp, Leisha M, Bruinsma, Rica, Vogel, Kurt W

“…The mammalian target of rapamycin (mTOR) is a serine/threonine kinase involved in nutrient sensing and cell growth and is a validated target for oncology and…”
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Multiplexing fluorescence polarization assays to increase information content per screen: applications for screening steroid hormone receptors by Blommel, Paul, Hanson, George T, Vogel, Kurt W

“…As the push to reduce cost per well in high-throughput screening reaches the practical limitations of liquid handling, future cost savings will likely arise…”
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