Degradation of nucleic acids during generation of superoxide-anion in the presence of copper ions

The efficiency of Escherichia coli nucleic acids samples: covalently closed circular DNA, linear chromosomal DNA, total RNA degradation mediated by the action of high oxygen pressure; hydrochloric hydroxylamine in alkaline conditions in the presence of cooper ions and in analogous conditions without...

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Bibliographic Details
Published in:	Molekuliarnaia biologiia Vol. 21; no. 6; p. 1664
Main Authors:	Vodolazkin, D I, Chistiakov, V A, Gus'kov, E P, Sherstnev, K B
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1987
Subjects:	Copper - pharmacology DNA - drug effects DNA - metabolism DNA Damage DNA, Bacterial - drug effects DNA, Bacterial - metabolism DNA, Circular - drug effects DNA, Circular - metabolism DNA, Single-Stranded - drug effects DNA, Single-Stranded - metabolism DNA, Superhelical - drug effects DNA, Superhelical - metabolism Escherichia coli - metabolism Nucleic Acid Conformation RNA - drug effects RNA - metabolism RNA, Bacterial - drug effects RNA, Bacterial - metabolism Superoxides - metabolism
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Summary:	The efficiency of Escherichia coli nucleic acids samples: covalently closed circular DNA, linear chromosomal DNA, total RNA degradation mediated by the action of high oxygen pressure; hydrochloric hydroxylamine in alkaline conditions in the presence of cooper ions and in analogous conditions without cooper ions was studied. The nativity of nucleic acids was determined by means of fluorometric analysis of nucleic acids/ethidium bromide complexes. Experiments revealed, that the destructive effect of active oxygen species decreased in the following order: NH2OH.HCl in alkaline conditions in the presence of copper ions-NH2.HCl in alkaline conditions--high pressure of pure oxygen. The stability of nucleic acids decreased in the following order: covalently closed circular DNA-linear DNA-RNA.
ISSN:	0026-8984