Spinochrome Identification and Quantification in Pacific Sea Urchin Shells, Coelomic Fluid and Eggs Using HPLC-DAD-MS

The high-performance liquid chromatography method coupled with diode array and mass spectrometric detector (HPLC-DAD-MS) method for quinonoid pigment identification and quantification in sea urchin samples was developed and validated. The composition and quantitative ratio of the quinonoid pigments...

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Bibliographic Details
Published in:	Marine drugs Vol. 19; no. 1; p. 21
Main Authors:	Vasileva, Elena A, Mishchenko, Natalia P, Tran, Van T T, Vo, Hieu M N, Fedoreyev, Sergey A
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 06-01-2021 MDPI
Subjects:	Animal embryos Animals Chromatography Chromatography, High Pressure Liquid Climatic zones coelomic fluid Coelomic fluids Composition Echinarachnius parma Echinoidea Eggs Embryo, Nonmammalian Embryos Epidermis Epidermis - chemistry High performance liquid chromatography HPLC HPLC-DAD-MS Identification Laganum decagonale Liquid chromatography Maretia planulata Marine invertebrates Mass Spectrometry Membranes Ovum - chemistry Pacific Ocean Phyllacanthus imperialis Pigments Pigments, Biological Pluteus quantification Quinones - chemistry quinonoid pigments Scaphechinus griseus Scaphechinus mirabilis Sea urchins Sea Urchins - chemistry Sea Urchins - classification Sea Urchins - growth & development Sensors Shells Skin Spectrometry spinochromes Tropical climate Pacific Ocean Sea of Japan HPLC-DAD-MS eggs quantification coelomic fluid sea urchins embryos quinonoid pigments spinochromes
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Summary:	The high-performance liquid chromatography method coupled with diode array and mass spectrometric detector (HPLC-DAD-MS) method for quinonoid pigment identification and quantification in sea urchin samples was developed and validated. The composition and quantitative ratio of the quinonoid pigments of the shells of 16 species of sea urchins, collected in the temperate (Sea of Japan) and tropical (South-China Sea) climatic zones of the Pacific Ocean over several years, were studied. The compositions of the quinonoid pigments of sea urchins , , and were studied for the first time. A study of the composition of the quinonoid pigments of the coelomic fluid of ten species of sea urchins was conducted. The composition of quinonoid pigments of jelly-like egg membrane, of developing embryos and pluteus, was reported for the first time. In the case of , we have shown that the compositions of pigment granules of the shell epidermis, coelomic fluid, egg membrane, developing embryos and pluteus are different, which should enable a fuller understanding of the functions of pigments at different stages of life.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1660-3397 1660-3397
DOI:	10.3390/md19010021