Search Results - "Vlasov, Alexander P"

Disruption of a Calmodulin Central Helix-like Region of 10-Formyltetrahydrofolate Dehydrogenase Impairs Its Dehydrogenase Activity by Uncoupling the Functional Domains by Reuland, Steven N, Vlasov, Alexander P, Krupenko, Sergey A

“…10-Formyltetrahydrofolate dehydrogenase (FDH) is composed of three domains and possesses three catalytic activities but has only two catalytic centers. The…”
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Amyloid Fibril Formation of the Mouse VL Domain at Acidic pH by Martsev, Sergey P, Dubnovitsky, Anatoly P, Vlasov, Alexander P, Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

“…The recombinant VL domain that represents the variable part of the light chain (type κ) of mouse monoclonal antibody F11 directed against human spleen ferritin…”
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Modular organization of FDH: Exploring the basis of hydrolase catalysis by Reuland, Steven N., Vlasov, Alexander P., Krupenko, Sergey A.

“…An abundant enzyme of liver cytosol, 10‐formyltetrahydrofolate dehydrogenase (FDH), is an interesting example of a multidomain protein. It consists of two…”
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On the Role of Conserved Histidine 106 in 10-Formyltetrahydrofolate Dehydrogenase Catalysis by Krupenko, Sergey A., Vlasov, Alexander P., Wagner, Conrad

“…The enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH), converts 10-formyltetrahydrofolate (10-formyl-THF) to tetrahydrofolate in an NADP+-dependent…”
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Thermodynamic and functional characterization of a stable IgG conformer obtained by renaturation from a partially structured low pH-induced state by Martsev, Sergey P., Kravchuk, Zinaida I., Vlasov, Alexander P., Lyakhnovich, Georgy V.

“…At pH 2, rabbit IgG adopts a partially structured state that exhibits loss of thermal unfolding transition, tentatively assigned to the CH2 domain, whilst…”
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Antiferritin Single-Chain Fv Fragment Is a Functional Protein with Properties of a Partially Structured State:  Comparison with the Completely Folded VL Domain by Martsev, Sergey P, Chumanevich, Alexander A, Vlasov, Alexander P, Dubnovitsky, Anatoly P, Tsybovsky, Yaroslav I, Deyev, Sergey M, Cozzi, Anna, Arosio, Paolo, Kravchuk, Zinaida I

“…Differential scanning calorimetry and spectroscopic probes were applied to study folding and stability of the single-chain Fv fragment (scFv) of the anti-human…”
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Large increase in thermal stability of the CH2 domain of rabbit IgG after acid treatment as evidenced by differential scanning calorimetry by Martsev, S P, Kravchuk, Z I, Vlasov, A P

“…Rabbit IgG after exposure to 0.05 M glycine-HCl, pH 2.0, and native IgG were compared by differential scanning calorimetry (DSC) at pH 3.5 and C1q binding…”
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On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis: connection between hydrolase and dehydrogenase mechanisms by Krupenko, S A, Vlasov, A P, Wagner, C

“…The enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH), converts 10-formyltetrahydrofolate (10-formyl-THF) to tetrahydrofolate in an NADP(+)-dependent…”
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Amyloid Fibril Formation of the Mouse V L Domain at Acidic pH by Martsev, Sergey P., Dubnovitsky, Anatoly P., Vlasov, Alexander P., Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

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Antiferritin single-chain Fv fragment is a functional protein with properties of a partially structured state: comparison with the completely folded V(L) domain by Martsev, S P, Chumanevich, A A, Vlasov, A P, Dubnovitsky, A P, Tsybovsky, Y I, Deyev, S M, Cozzi, A, Arosio, P, Kravchuk, Z I

“…Differential scanning calorimetry and spectroscopic probes were applied to study folding and stability of the single-chain Fv fragment (scFv) of the anti-human…”
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Amyloid fibril formation of the mouse V(L) domain at acidic pH by Martsev, Sergey P, Dubnovitsky, Anatoly P, Vlasov, Alexander P, Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

“…The recombinant V(L) domain that represents the variable part of the light chain (type kappa) of mouse monoclonal antibody F11 directed against human spleen…”
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Antiferritin Single-Chain Fv Fragment Is a Functional Protein with Properties of a Partially Structured State:  Comparison with the Completely Folded V L Domain by Martsev, Sergey P., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Tsybovsky, Yaroslav I., Deyev, Sergey M., Cozzi, Anna, Arosio, Paolo, Kravchuk, Zinaida I.

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Distinct stability of recombinant L and H subunits of human ferritin: calorimetric and ANS binding studies by Martsev, S P, Vlasov, A P, Arosio, P

“…Thermodynamic and pH stability of recombinant human L- and H-ferritins were probed by differential scanning calorimetry and 8-anilino-1-naphthalenesulfonate…”
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Antiferritin single-chain antibody: a functional protein with incomplete folding? by Martsev, Sergey P., Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Bespalov, Ivan A., Arosio, Paolo, Deyev, Sergey M.

“…The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB…”
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Two high-affinity monoclonal IgG2a antibodies with differing thermodynamic stability demonstrate distinct antigen-induced changes in protein A-binding affinity by Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Martsev, Sergey P.

“…Two IgG2a monoclonal antibodies (G10 and F11) are described which have similar affinity for human spleen ferritin and identical protein A-binding affinity. The…”
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