Quantitative and qualitative analysis of pulmonary arterial hypertension fibrosis using wide-field second harmonic generation microscopy

Quantitative and qualitative analysis of pulmonary arterial hypertension fibrosis using wide-field second harmonic generation microscopy

We demonstrated that wide-field second harmonic generation (SHG) microscopy of lung tissue in combination with quantitative analysis of SHG images is a powerful tool for fast and label-free visualization of the fibrosis pathogenesis in pulmonary arterial hypertension (PAH). Statistical analysis of t...

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Bibliographic Details
Published in:Scientific reports Vol. 12; no. 1; p. 7330
Main Authors: Padrez, Yaraslau, Golubewa, Lena, Kulahava, Tatsiana, Vladimirskaja, Tatyana, Semenkova, Galina, Adzerikho, Igor, Yatsevich, Olga, Amaegberi, Nadezda, Karpicz, Renata, Svirko, Yuri, Kuzhir, Polina, Rutkauskas, Danielis
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 05-05-2022
Nature Publishing Group
Nature Portfolio
Subjects:
631/57
639/624
692/1807
692/308
692/699
Alveoli
Animals
Blood vessels
Collagen
Fibrosis
Fourier Analysis
Humanities and Social Sciences
Hypertension
Lung cancer
Lung diseases
Microscopy
Monocrotaline
multidisciplinary
Pulmonary Arterial Hypertension
Pulmonary hypertension
Qualitative analysis
Rats
Science
Science (multidisciplinary)
Second Harmonic Generation Microscopy - methods
Statistical analysis
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Summary:We demonstrated that wide-field second harmonic generation (SHG) microscopy of lung tissue in combination with quantitative analysis of SHG images is a powerful tool for fast and label-free visualization of the fibrosis pathogenesis in pulmonary arterial hypertension (PAH). Statistical analysis of the SHG images revealed changes of the collagen content and morphology in the lung tissue during the monocrotaline-induced PAH progression in rats. First order statistics disclosed the dependence of the collagen overproduction on time, the second order statistics indicated tightening of collagen fiber network around blood vessels and their spreading into the alveolar region. Fourier analysis revealed that enhancement of the fiber orientation in the collagen network with PAH progression was followed with its subsequent reduction at the terminating phase of the disease. Proposed approach has potential for assessing pulmonary fibrosis in interstitial lung disease, after lung(s) transplantation, cancer, etc.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-11473-5