Search Results - "Viswanatha, Raghuvir"

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells by Viswanatha, Raghuvir, Li, Zhongchi, Hu, Yanhui, Perrimon, Norbert

“…Genome-wide screens in cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large…”
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Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos by Viswanatha, Raghuvir, Mameli, Enzo, Rodiger, Jonathan, Merckaert, Pierre, Feitosa-Suntheimer, Fabiana, Colpitts, Tonya M., Mohr, Stephanie E., Hu, Yanhui, Perrimon, Norbert

“…Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured…”
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CRISPR guide RNA design for research applications by Mohr, Stephanie E., Hu, Yanhui, Ewen‐Campen, Benjamin, Housden, Benjamin E., Viswanatha, Raghuvir, Perrimon, Norbert

“…The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools…”
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Author Correction: Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos by Viswanatha, Raghuvir, Mameli, Enzo, Rodiger, Jonathan, Merckaert, Pierre, Feitosa-Suntheimer, Fabiana, Colpitts, Tonya M., Mohr, Stephanie E., Hu, Yanhui, Perrimon, Norbert

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Dynamics of ezrin and EBP50 in regulating microvilli on the apical aspect of epithelial cells by Viswanatha, Raghuvir, Bretscher, Anthony, Garbett, Damien

“…Microvilli are found on the apical surface of epithelial cells. Recent studies on the microvillar proteins ezrin and EBP50 (ezrin/radixin/moesin-binding…”
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Effector-mediated ERM activation locally inhibits RhoA activity to shape the apical cell domain by Zaman, Riasat, Lombardo, Andrew, Sauvanet, Cécile, Viswanatha, Raghuvir, Awad, Valerie, Bonomo, Locke Ezra-Ros, McDermitt, David, Bretscher, Anthony

“…Activated ezrin-radixin-moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton to generate apical structures, including microvilli. Among…”
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CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells by Xia, Baolong, Amador, Gabriel, Viswanatha, Raghuvir, Zirin, Jonathan, Mohr, Stephanie E., Perrimon, Norbert

“…Precise and efficient genome modifications provide powerful tools for biological studies. Previous CRISPR gene knockout methods in cell lines have relied on…”
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Local phosphocycling mediated by LOK/SLK restricts ezrin function to the apical aspect of epithelial cells by Viswanatha, Raghuvir, Ohouo, Patrice Y, Smolka, Marcus B, Bretscher, Anthony

“…In this paper, we describe how a dynamic regulatory process is necessary to restrict microvilli to the apical aspect of polarized epithelial cells. We found…”
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Ezrin activation by LOK phosphorylation involves a PIP2-dependent wedge mechanism by Pelaseyed, Thaher, Viswanatha, Raghuvir, Sauvanet, Cécile, Filter, Joshua J, Goldberg, Michael L, Bretscher, Anthony

“…How cells specify morphologically distinct plasma membrane domains is poorly understood. Prior work has shown that restriction of microvilli to the apical…”
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Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells by Xia, Baolong, Viswanatha, Raghuvir, Hu, Yanhui, Mohr, Stephanie E, Perrimon, Norbert

“…Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens…”
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SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing by Chen, Chiao-Lin, Rodiger, Jonathan, Chung, Verena, Viswanatha, Raghuvir, Mohr, Stephanie E, Hu, Yanhui, Perrimon, Norbert

“…CRISPR-Cas9 is a powerful genome editing technology in which a single guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome…”
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A genome-wide CRISPR screen identifies DPM1 as a modifier of DPAGT1 deficiency and ER stress by Dalton, Hans M, Viswanatha, Raghuvir, Brathwaite, Jr, Roderick, Zuno, Jae Sophia, Berman, Alexys R, Rushforth, Rebekah, Mohr, Stephanie E, Perrimon, Norbert, Chow, Clement Y

“…Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In…”
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Interactome Analysis Reveals Ezrin Can Adopt Multiple Conformational States by Viswanatha, Raghuvir, Wayt, Jessica, Ohouo, Patrice Y., Smolka, Marcus B., Bretscher, Anthony

“…Ezrin, a member of the ezrin-radixin-moesin family (ERM), is an essential regulator of the structure of microvilli on the apical aspect of epithelial cells…”
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A Block in Endoplasmic Reticulum-to-Golgi Trafficking Inhibits Phospholipid Synthesis and Induces Neutral Lipid Accumulation by Gaspar, Maria L., Jesch, Stephen A., Viswanatha, Raghuvir, Antosh, Amy L., Brown, William J., Kohlwein, Sepp D., Henry, Susan A.

“…Seeking to better understand how membrane trafficking is coordinated with phospholipid synthesis in yeast, we investigated lipid synthesis in several Sec-…”
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State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila by Zirin, Jonathan, Bosch, Justin, Viswanatha, Raghuvir, Mohr, Stephanie E., Perrimon, Norbert

“…For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many…”
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CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor by Xu, Ying, Viswanatha, Raghuvir, Sitsel, Oleg, Roderer, Daniel, Zhao, Haifang, Ashwood, Christopher, Voelcker, Cecilia, Tian, Songhai, Raunser, Stefan, Perrimon, Norbert, Dong, Min

“…Entomopathogenic nematodes are widely used as biopesticides 1 , 2 . Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens…”
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A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila by Okamoto, Naoki, Viswanatha, Raghuvir, Bittar, Riyan, Li, Zhongchi, Haga-Yamanaka, Sachiko, Perrimon, Norbert, Yamanaka, Naoki

“…Steroid hormones are a group of lipophilic hormones that are believed to enter cells by simple diffusion to regulate diverse physiological processes through…”
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Local assembly of long reads enables phylogenomics of transposable elements in a polyploid cell line by Han, Shunhua, Dias, Guilherme B, Basting, Preston J, Viswanatha, Raghuvir, Perrimon, Norbert, Bergman, Casey M

“…Animal cell lines often undergo extreme genome restructuring events, including polyploidy and segmental aneuploidy that can impede de novo whole-genome…”
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The tails of apical scaffolding proteins EBP50 and E3KARP regulate their localization and dynamics by Garbett, Damien, Sauvanet, Cécile, Viswanatha, Raghuvir, Bretscher, Anthony

“…The closely related apical scaffolding proteins ERM-binding phosphoprotein of 50 kDa (EBP50) and NHE3 kinase A regulatory protein (E3KARP) both consist of two…”
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Pooled CRISPR Screens in Drosophila Cells by Viswanatha, Raghuvir, Brathwaite, Roderick, Hu, Yanhui, Li, Zhongchi, Rodiger, Jonathan, Merckaert, Pierre, Chung, Verena, Mohr, Stephanie E, Perrimon, Norbert

“…High-throughput screens in Drosophila melanogaster cell lines have led to discovery of conserved gene functions related to signal transduction, host-pathogen…”
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