The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice

The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice

Abstract To improve skin flap healing, one promising strategy in reconstructive surgery might be to optimize platelet rich plasma (PRP) bioactivity and the ischemia-altered expression of genes. We studied both the effect of PRP on ischemic flaps, and whether in vivo bioluminescence imaging (BLI) is...

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Published in:Biomaterials Vol. 34; no. 11; pp. 2674 - 2682
Main Authors: Sönmez, Tolga Taha, Vinogradov, Alexandra, Zor, Fatih, Kweider, Nisreen, Lippross, Sebastian, Liehn, Elisa Anamaria, Naziroglu, Mustafa, Hölzle, Frank, Wruck, Christoph, Pufe, Thomas, Tohidnezhad, Mersedeh
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01-04-2013
Subjects:
Advanced Basic Science
Animals
Bioluminescence imaging
Cell Survival
Dentistry
Enzyme-Linked Immunosorbent Assay
Free flap
Gene Expression
Immunohistochemistry
Ischemia
Ischemia - pathology
Luminescent Measurements
Mice
Mice, Transgenic
Neovascularization, Physiologic
Platelet rich plasma
Platelet-Rich Plasma - metabolism
Surgical Flaps - blood supply
Vascular Endothelial Growth Factor Receptor-2 - genetics
Vascular Endothelial Growth Factor Receptor-2 - metabolism
VEGF
VEGFR2
Wound Healing
VEGFR2
Platelet rich plasma
Bioluminescence imaging
Ischemia
VEGF
Free flap
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Summary:Abstract To improve skin flap healing, one promising strategy in reconstructive surgery might be to optimize platelet rich plasma (PRP) bioactivity and the ischemia-altered expression of genes. We studied both the effect of PRP on ischemic flaps, and whether in vivo bioluminescence imaging (BLI) is a suitable method for the longitudinal monitoring of angiogenesis in surgical wounds. Axial murine skin flaps were created in four experimental groups. In vivo measurements of VEGFR2 expression levels were made every other day until the 14th day. The local VEGF level and microvessel density were quantified on the 14th day via ELISA and immunohistochemistry, and flap survival rates were measured. We demonstrated that PRP and induced ischemia have a beneficial influence on angiogenesis and flap healing. Combining the two resulted in a significantly robust increase in angiogenesis and flap survival rate that was corroborated by bioluminescence imaging of VEGFR2 activity. This study shows that angiogenic effects of PRP may be potentialized by the stimulus of induced ischemia during free flap harvesting, and thus the two procedures appear to have a synergistic effect on flap healing. This study further demonstrates that BLI of modulated genes in reconstructive surgery is a valuable model for longitudinal in vivo evaluation of angiogenesis.
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.01.016