Spineless and overlooked: DNA metabarcoding of autonomous reef monitoring structures reveals intra‐ and interspecific genetic diversity in Mediterranean invertebrates

Spineless and overlooked: DNA metabarcoding of autonomous reef monitoring structures reveals intra‐ and interspecific genetic diversity in Mediterranean invertebrates

The ability to gather genetic information using DNA metabarcoding of bulk samples obtained directly from the environment is crucial to determine biodiversity baselines and understand population dynamics in the marine realm. While DNA metabarcoding is effective in evaluating biodiversity at community...

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Bibliographic Details
Published in:Molecular ecology resources Vol. 23; no. 7; pp. 1689 - 1705
Main Authors: Thomasdotter, Anna, Shum, Peter, Mugnai, Francesco, Vingiani, Marina, Dubut, Vincent, Marschal, Florent, Abbiati, Marco, Chenuil, Anne, Costantini, Federica
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-10-2023
Wiley/Blackwell
Subjects:
Animal genetics
autonomous reef monitoring structures
Biodiversity
Biodiversity and Ecology
Bioinformatics
COI
Deoxyribonucleic acid
DNA
DNA barcoding
DNA metabarcoding
Earth Sciences
Environmental Sciences
Genetic analysis
Genetic diversity
Genetic variability
Genetics
haplotype diversity
Haplotypes
invertebrate
Invertebrates
Life Sciences
Marine invertebrates
Mediterranean Sea
Monitoring
Oceanography
Population dynamics
Population genetics
Populations and Evolution
Sciences of the Universe
Species
Species diversity
Taxa
autonomous reef monitoring structures
haplotype diversity
invertebrate
COI
DNA metabarcoding
Mediterranean Sea
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Summary:The ability to gather genetic information using DNA metabarcoding of bulk samples obtained directly from the environment is crucial to determine biodiversity baselines and understand population dynamics in the marine realm. While DNA metabarcoding is effective in evaluating biodiversity at community level, genetic patterns within species are often concealed in metabarcoding studies and overlooked for marine invertebrates. In the present study, we implement recently developed bioinformatics tools to investigate intraspecific genetic variability for invertebrate taxa in the Mediterranean Sea. Using metabarcoding samples from Autonomous Reef Monitoring Structures (ARMS) deployed in three locations, we present haplotypes and diversity estimates for 145 unique species. While overall genetic diversity was low, we identified several species with high diversity records and potential cryptic lineages. Further, we emphasize the spatial scale of genetic variability, which was observed from locations to individual sampling units (ARMS). We carried out a population genetic analysis of several important yet understudied species, which highlights the current knowledge gap concerning intraspecific genetic patterns for the target taxa in the Mediterranean basin. Our approach considerably enhances biodiversity monitoring of charismatic and understudied Mediterranean species, which can be incorporated into ARMS surveys.
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ISSN:1755-098X
1755-0998
DOI:10.1111/1755-0998.13836