Hydrogen and polyhydroxybutyrate production from wheat straw hydrolysate using Caldicellulosiruptor species and Ralstonia eutropha in a coupled process

•Caldicellulosiruptor spp. produced 134 mmol H2/L from wheat straw hydrolysate.•Side stream of H2 production used as C source for PHB synthesis by R. eutropha.•Highest PHB content of ∼83% was obtained at 20% aeration and nitrogen depletion.•41.5% of carbon and 51.9% of electrons used for PHB product...

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Bibliographic Details
Published in:	Bioresource technology Vol. 272; pp. 259 - 266
Main Authors:	Soto, Luis Romero, Byrne, Eoin, van Niel, Ed W.J., Sayed, Mahmoud, Villanueva, Cristhian Carrasco, Hatti-Kaul, Rajni
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-01-2019
Subjects:	Bioenergi Bioenergy Caldicellulosiruptor species Cupriavidus necator - metabolism Engineering and Technology Firmicutes - metabolism Glucose - metabolism Hydrogen Hydrogen - metabolism Hydrolysis Hydroxybutyrates - metabolism Industrial Biotechnology Industriell bioteknik Integrated biorefinery Polyhydroxybutyrate Ralstonia eutropha Teknik Triticum - metabolism Wheat straw hydrolysate Xylose - metabolism Wheat straw hydrolysate Ralstonia eutropha Hydrogen Polyhydroxybutyrate Integrated biorefinery Caldicellulosiruptor species
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Summary:	•Caldicellulosiruptor spp. produced 134 mmol H2/L from wheat straw hydrolysate.•Side stream of H2 production used as C source for PHB synthesis by R. eutropha.•Highest PHB content of ∼83% was obtained at 20% aeration and nitrogen depletion.•41.5% of carbon and 51.9% of electrons used for PHB production in coupled process. This report presents an integrated biorefinery concept in which wheat straw hydrolysate was treated with co-cultures of osmotolerant thermophilic bacterial strains, Caldicellulosiruptor saccharolyticus and C. owensensis to obtain hydrogen, while the liquid effluent containing acetate and residual glucose was used as feed for polyhydroxybutyrate (PHB) production by Ralstonia eutropha. The Caldicellulosiruptor spp. co-culture consumed 10.8 g/L of pretreated straw sugars, glucose and xylose, producing 134 mmol H2/L. PHB accumulation by R. eutropha was first studied in minimal salts medium using acetate with/without glucose as carbon source. Addition of salts promoted cell growth and PHB production in the effluent. Fed-batch cultivation in a nitrogen limited medium with 40% (v/v) aeration resulted in a cell density of 15.1 g/L with PHB content of 80.1% w/w and PHB concentration of 12.1 g/L, while 20% aeration gave a cell density of 11.3 g/L with 83.4% w/w PHB content and 9.4 g/L PHB concentration.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0960-8524 1873-2976
DOI:	10.1016/j.biortech.2018.09.142