The Effect of Amphotericin B on the Viability of Cryopreserved Human Skin

To evaluate whether amphotericin B has a detrimental effect on the viability of cryopreserved skin, human skin samples were incubated during 7 and 24 h in three different media: 0.9% phosphate-buffered saline and Medium 199 with and without 10 μg/ml amphotericin B. The viability of the tissue was as...

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Bibliographic Details
Published in:	Cryobiology Vol. 32; no. 4; pp. 314 - 317
Main Authors:	Villalba, Rafael, Alonso, Pedro, Villalba, J.Manuel, Rioja, Luis F., Villagrán, J.Luis Gómez
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 01-08-1995
Subjects:	Amphotericin B - adverse effects Cell Survival - drug effects Cryopreservation - methods Culture Media Female Humans In Vitro Techniques Middle Aged NADPH-Ferrihemoprotein Reductase - metabolism Skin - cytology Skin - drug effects Skin - enzymology
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Summary:	To evaluate whether amphotericin B has a detrimental effect on the viability of cryopreserved skin, human skin samples were incubated during 7 and 24 h in three different media: 0.9% phosphate-buffered saline and Medium 199 with and without 10 μg/ml amphotericin B. The viability of the tissue was assessed by measuring the tetrazolium reductase activity before and after cryopreservation. A significant decrease in cryopreserved human skin viability was observed after 7 and 24 h incubation with amphotericin B. These results indicate that in the processing of skin preservation, the beneficial effect of using amphotericin B as an antifungal agent may be reduced by its negative effect on tissue viability.
ISSN:	0011-2240 1090-2392
DOI:	10.1006/cryo.1995.1031