Search Results - "Villain, Louis"

Purification of Monoclonal Antibodies Using a Fiber Based Cation-Exchange Stationary Phase: Parameter Determination and Modeling by Schwellenbach, Jan, Zobel, Steffen, Taft, Florian, Villain, Louis, Strube, Jochen

“…Monoclonal antibodies (mAb) currently dominate the market for protein therapeutics. Because chromatography unit operations are critical for the purification of…”
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Preparation and characterization of high capacity, strong cation-exchange fiber based adsorbents by Schwellenbach, Jan, Taft, Florian, Villain, Louis, Strube, Jochen

“…•Novel fiber based cation-exchange adsorbers have been produced by SI-ATRP.•Favorable hydrogel and packed bed structures could be generated.•High volumetric…”
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Continuous purification of influenza A virus particles using pseudo-affinity membrane chromatography by Fortuna, A. Raquel, Taft, Florian, Villain, Louis, Wolff, Michael W., Reichl, Udo

“…Robust and flexible continuous unit operations that enable the establishment of intensified bioprocesses is one of the most relevant trends in manufacturing of…”
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Use of sulfated cellulose membrane adsorbers for chromatographic purification of cell cultured-derived influenza A and B viruses by Fortuna, A. Raquel, van Teeffelen, Sebastian, Ley, Adrian, Fischer, Laura M., Taft, Florian, Genzel, Yvonne, Villain, Louis, Wolff, Michael W., Reichl, Udo

“…•Sulfated matrices are suitable for the purification of influenza virus particles.•Membranes adsorbers show higher productivity compared to resins.•Membrane…”
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Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles by Ladd Effio, Christopher, Hahn, Tobias, Seiler, Julia, Oelmeier, Stefan A., Asen, Iris, Silberer, Christine, Villain, Louis, Hubbuch, Jürgen

“…•Purification of human B19 parvovirus-like particles by anion-exchange membrane chromatography.•High-throughput screening-based selection of VLP binding…”
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Purification of bone morphogenetic protein-2 from refolding mixtures using mixed-mode membrane chromatography by Gieseler, Gesa, Pepelanova, Iliyana, Stuckenberg, Lena, Villain, Louis, Nölle, Volker, Odenthal, Uwe, Beutel, Sascha, Rinas, Ursula, Scheper, Thomas

“…In this study, we present the development of a process for the purification of recombinant human bone morphogenetic protein-2 (rhBMP-2) using mixed-mode…”
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Controlling the polymer-nanolayer architecture on anion-exchange membrane adsorbers via surface-initiated atom transfer radical polymerization by Schwellenbach, Jan, Kosiol, Peter, Sölter, Björn, Taft, Florian, Villain, Louis, Strube, Jochen

“…The immobilization of a polymer-nanolayer containing ligand sites is a widely used approach to increase the binding capacity of membrane adsorbers. In this…”
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Optimization of cell culture‐derived influenza A virus particles purification using sulfated cellulose membrane adsorbers by Fortuna, Ana Raquel, Taft, Florian, Villain, Louis, Wolff, Michael W., Reichl, Udo

“…Downstream processing remains one of the biggest challenges in manufacturing of biologicals and vaccines. This work focuses on a Design of Experiments approach…”
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Single-component adsorption of proteins on a cellulose membrane with the phenyl ligand for hydrophobic interaction chromatography by Kosior, Anna, Antošová, Monika, Faber, Rene, Villain, Louis, Polakovič, Milan

“…Porous structure and adsorption properties of a hydrophobic interaction chromatography adsorbent, Sartobind PhenylTM, were studied using bovine γ-globulin,…”
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Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles by Nestola, Piergiuseppe, Peixoto, Cristina, Villain, Louis, Alves, Paula M., Carrondo, Manuel J.T., Mota, José P.B.

“…•Flow-through purification of virus vectors and virus-like particles is demonstrated.•Different flow-through platforms were developed for adenovirus type-5 and…”
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One-step-purification of penicillin G amidase from cell lysate using ion-exchange membrane adsorbers by Mönster, Andrea, Villain, Louis, Scheper, Thomas, Beutel, Sascha

“…This study describes the purification of penicillin G amidase (PGA) by ion exchange membrane adsorbers in a one-step-process. Preliminary experiments with…”
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Impact of grafting on the design of new membrane adsorbers for adenovirus purification by Nestola, Piergiuseppe, Villain, Louis, Peixoto, Cristina, Martins, Duarte L., Alves, Paula M., Carrondo, Manuel J.T., Mota, José P.B.

“…•Hydrogel grafted membranes are more suitable for virus purification than direct grafted ones.•Virus recovery is better for hydrogel grafted ligand densities…”
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Development of a Novel Membrane Aerated Hollow-Fiber Microbioreactor by Villain, Louis, Meyer, Lina, Kroll, Stephen, Beutel, Sascha, Scheper, Thomas

“…A new challenge in biotechnological processes is the development of flexible bioprocessing platforms, allowing strain selection, facilitating scale‐up and…”
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Development of a Novel Membrane Aerated Hollow-Fiber Microbioreactor by Villain, Louis, Meyer, Lina, Kroll, Stephen, Beutel, Sascha, Scheper, Thomas

“…A new challenge in biotechnological processes is the development of flexible bioprocessing platforms, allowing strain selection, facilitating scale-up and…”
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