Search Results - "Viljoen, GJ"

Protective immune responses induced by different recombinant vaccine regimes to Rift Valley fever by Wallace, D.B., Ellis, C.E., Espach, A., Smith, S.J., Greyling, R.R., Viljoen, G.J.

“…The glycoprotein (GP) and nucleocapsid (NC) genes of Rift Valley fever virus (RVFV) were expressed in different expression systems and were evaluated for their…”
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Immune responses to recombinants of the South African vaccine strain of lumpy skin disease virus generated by using thymidine kinase gene insertion by Wallace, David B., Viljoen, Gerrit J.

“…The South African vaccine strain of lumpy skin disease virus (type SA-Neethling) is currently being developed as a vector for recombinant vaccines of…”
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A phylogenetic study of South African Newcastle disease virus strains isolated between 1990 and 2002 suggests epidemiological origins in the Far East by ABOLNIK, C, HORNER, R. F, BISSCHOP, S. P. R, PARKER, M. E, ROMITO, M, VILJOEN, G. J

“…Genetic comparisons were made of the fusion protein sequences of 155 Newcastle disease virus isolates collected in South Africa between 1990 and 2002. Their…”
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Comparative sequence analysis of the South African vaccine strain and two virulent field isolates of Lumpy skin disease virus by KARA, P. D, AFONSO, C. L, ROCK, D. L, WALLACE, D. B, KUTISH, G. F, ABOLNIK, C, LU, Z, VREEDE, F. T, TALJAARD, L. C. F, ZSAK, A, VILJOEN, G. J

“…The genomic sequences of 3 strains of Lumpy skin disease virus (LSDV) (Neethling type) were compared to determine molecular differences, viz. the South African…”
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Inhibition of fowlpox virus by an aqueous acetone extract from galls of Guiera senegalensis J. F. Gmel (Combretaceae) by Lamien, Charles E., Meda, Aline, Mans, Janet, Romito, Marco, Nacoulma, Odile G., Viljoen, Gerrit J.

“…An aqueous acetone extract from the galls of Guiera senegalensis was screened for in vitro antiviral activity against fowlpox virus (FPV). Cytopathic effect…”
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Identification of further proteolytic cleavage sites in the Southampton calicivirus polyprotein by expression of the viral protease in E. coli by Liu, BL, Viljoen, GJ, Clarke, IN, Lambden, PR

“…BL Liu, GJ Viljoen, IN Clarke and PR Lambden Molecular Microbiology Group, University Medical School, Southampton General Hospital, UK. Southampton virus (SV)…”
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In ovo inhibition of fowlpoxvirus replication by a gall extract from Guiera senegalensis by Lemien, C.E, Mans, J, Meda, A, Couacy-Hymann, E, Romito, M, Ouedraogo, A.G, Nacoulma, O.G, Viljoen, G.J

“…Several field isolates of fowlpoxvirus (FPV) from Burkina Faso, West Africa, were isolated and partly evaluated by molecular analysis. In addition, the in ovo…”
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Single- tube nested PCR for detection of the sheep-associated agent of MCF by Dungu, B., Bosman, A-M., Kachelhoffer, C., Viljoen, G. J.

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Eliciting antigen-specific egg-yolk IgY with naked DNA by Romito, M, Viljoen, G J, Du Plessis, D H

“…Immunization with naked DNA was used to elicit chicken egg yolk antibodies (IgY). Layer hens were inoculated with plasmid DNA encoding the enhanced green…”
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Characterization of a pigeon paramyxovirus (PPMV-1) isolated from chickens in South Africa by Abolnik, C. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Research Inst.), Horner, P, Maharaj, R, Viljoen, G.J

“…A paramyxovirus with a thermostability of 60 min (typical of velogenic viruses) and a mean death time of > 90 h (typical of lentogenic viruses) was isolated…”
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PCR-based detection of the transovarial transmission of Uruguayan Babesia bovis and Babesia bigemina vaccine strains by Gayo, V, Romito, M, Nel, L H, Solari, M A, Viljoen, G J

“…Bovine babesiosis is responsible for serious economic losses in Uruguay. Haemovaccines play an important role in disease prevention, but concern has been…”
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Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR by Wang, Z, Vreede, F T, Mitchell, J O, Viljoen, G J

“…A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of…”
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Immune responses in a horse inoculated with the VP2 gene of African horsesickness virus by Romito, M, Du Plessis, D H, Viljoen, G J

“…The ability of a DNA vaccine to elicit an immune response in a horse was evaluated. The outer capsid protein VP2 of African horsesickness virus is known to…”
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Validation of an indirect enzyme-linked immunosorbent assay for the detection of antibody against Brucella abortus in cattle sera using an automated ELISA workstation by Paweska, J T, Potts, A D, Harris, H J, Smith, S J, Viljoen, G J, Dungu, B, Brett, O L, Bubb, M, Prozesky, L

“…An automated indirect enzyme-linked immunosorbent assay (I-ELISA) for the serological diagnosis of bovine brucellosis was developed and validated in-house. A…”
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Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation by Vreede, F.T., Cloete, M., Napier, G.B., van Dijk, A.A., Viljoen, G.J.

“…A strategy was developed for sequence-independent synthesis and amplification of full-length cDNA of 3–4 kb genes of dsRNA viruses. The method of single primer…”
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Identification and characterisation of an early/late bi-directional promoter of the capripoxvirus, lumpy skin disease virus by Fick, W.C, Viljoen, G.J

“…Identification and characterisation of an early/late bi-directional promoter element of lumpy skin disease virus (LSDV) is described. The 56 bp element shows…”
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Detection of African horsesickness virus and discrimination between two equine orbivirus serogroups by reverse transcription polymerase chain reaction by Bremer, C W, Viljoen, G J

“…A reverse transcription polymerase chain reaction (RT-PCR), based on the gene encoding the NS2 protein of African horsesickness virus (AHSV), was developed for…”
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The Characterization of Equine Encephalosis Virus and the Development of Genomic Probes by Viljoen, G. J, Huismans, H

“…1 Department of Biochemistry, Veterinary Research Institute, Onderstepoort 0110 and 2 Department of Genetics, University of Pretoria, Pretoria 0002, South…”
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Early and late transcriptional phases in the replication of lumpy-skin-disease virus by Fick, W C, Viljoen, G J

“…Approximately 71 of the estimated 145 kilobase pairs of the genome of the South African isolate of lumpy-skin-disease virus, Neethling strain, was cloned into…”
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Direct identification of recombinant vaccinia virus plaques by PCR by Pasamontes, L, Gubser, J, Wittek, R, Viljoen, G J

“…A fast method for the identification of recombinant vaccinia viruses directly from individual plaques is described. Plaques are picked, resuspended in PBS-A…”
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