Search Results - "Vierstra, R.D."

Post-translational regulation in plants employing a diverse set of polypeptide tags by Downes, B, Vierstra, R D

“…The concept that plants exploit polypeptides as post-translational modifiers is rapidly emerging as an important method to manipulate various cellular…”
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Signaling among neighboring plants and the development of size inequalities in plant populations by Ballaré, Carlos L., Scopel, Ana L., Jordan, Emily T., Vierstra, Richard D.

“…Transgenic tobacco plants that express an oat phytochrome gene (phyA) under control of the cauliflower mosaic virus (CaMV) 35S promoter and display altered…”
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Three-dimensional structure of a ubiquitin-conjugating enzyme (E2) by COOK, W. J, JEFFREY, L. C, SULLIVAN, M. L, VIERSTRA, R. D

“…The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined…”
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Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways by Thompson, A.R, Doelling, J.H, Suttangkakul, A, Vierstra, R.D

“…Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then…”
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Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis thaliana. Identification of functional domains by in vitro mutagenesis by Sullivan, M.L, Vierstra, R.D

“…Ubiquitin carrier proteins (E2s) are involved in the covalent attachment of ubiquitin to a variety of cellular target proteins in eukaryotes. Here, we report…”
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A major ubiquitin conjugation system in wheat germ extracts involves a 15-kDa ubiquitin-conjugating enzyme (E2) homologous to the yeast UBC4/UBC5 gene products by Girod, P.A, Vierstra, R.D

“…In eukaryotes, conjugation of ubiquitin to proteins serves as a committed step for intracellular protein degradation. Formation of ubiquitin-protein conjugates…”
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Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis by Hatfield, P.M. (University of Wisconsin, Madison, WI), Vierstra, R.D

“…Ubiquitin-activating enzyme, E1, directs the ATP-dependent formation of a thiol ester linkage between itself and ubiquitin. The energy in this bond is…”
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Autophagic recycling: lessons from yeast help define the process in plants by Thompson, Allison R, Vierstra, Richard D

“…The autophagic engulfment of cytoplasm and organelles and their delivery to the vacuole have long been speculated to play an essential role in bulk protein…”
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The Exoribonuclease XRN4 Is a Component of the Ethylene Response Pathway in Arabidopsis by Potuschak, Thomas, Vansiri, Amérin, Binder, Brad M., Lechner, Esther, Vierstra, Richard D., Genschik, Pascal

“…EXORIBONUCLEASE4 (XRN4), the Arabidopsis thaliana homolog of yeast XRN1, is involved in the degradation of several unstable mRNAs. Although a role for XRN4 in…”
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Characterization of tobacco expressing functional oat phytochrome. Domains responsible for the rapid degradation of Pfr are conserved between monocots and dicots by Cherry, J.R. (University of Wisconsin-Madison, Madison, WI), Hershey, H.P, Vierstra, R.D

“…Constitutive expression of a chimeric oat phytochrome gene in tobacco (Nicotiana tabacum) results in the accumulation of a functional 124-kilodalton…”
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Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation by Gagne, J.M, Smalle, J, Gingerich, D.J, Walker, J.M, Yoo, S.D, Yanagisawa, S, Vierstra, R.D

“…Ubiquitination of various intracellular proteins by ubiquitin-protein ligases (or E3s) plays an essential role in eukaryotic cell regulation primarily through…”
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High-irradiance responses induced by far-red light in grass seedlings of the wild type or overexpressing phytochrome A by Casal, J.J, Clough, R.C, Vierstra, R.D. (Buenos Aires Univ. (Argentina). Dept. de Ecologia)

“…The occurrence of phytochrome-mediated high-irradiance responses (HIR), previously characterised largely in dicotyledonous plants, was investigated in Triticum…”
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Cloning of ubiquitin activating enzyme from wheat and expression of a functional protein in Escherichia coli by Hatfield, P.M. (University of Wisconsin, Madison, WI), Callis, J, Vierstra, R.D

“…The initial step in the conjugation of ubiquitin to substrate proteins involves the activation of ubiquitin by ubiquitin activating enzyme, E1. Previously, we…”
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pair of bacteriophytochromes from Agrobacterium tumefaciens are histidine kinases with opposing photobiological properties by Karniol, B, Vierstra, R.D

“…Bacteriophytochrome photoreceptors (BphPs) are a family of phytochrome-like sensor kinases that help a wide variety of bacteria respond to their light…”
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Comparison of the three-dimensional structures of human, yeast, and oat ubiquitin by Vijay-Kumar, S., Bugg, C.E., Wilkinson, K.D., Vierstra, R.D., Hatfield, P.M., Cook, W.J.

“…The crystal structure of human ubiquitin has been solved by x-ray diffraction methods and refined by standard procedures to a conventional crystallographic R…”
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Formation of a stable adduct between ubiquitin and the Arabidopsis ubiquitin-conjugating enzyme, AtUBC1 by Sullivan, M.L, Vierstra, R.D

“…Ubiquitin conjugating enzymes (E2s) are an integral part of a multienzyme pathway that ligates ubiquitin to intracellular target proteins. This ligation has…”
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Hemin inhibits ubiquitin-dependent proteolysis in both a higher plant and yeast by Vierstra, Richard D, Sullivan, Michael L

“…In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the…”
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Mass spectrometric resolution of reversible protein phosphorylation in photosynthetic membranes of Arabidopsis thaliana by Vener, A V, Harms, A, Sussman, M R, Vierstra, R D

“…The use of mass spectrometry to characterize the phosphorylome, i.e. the constituents of the proteome that become phosphorylated, was demonstrated using the…”
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Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants by Vierstra, R.D. (Wisconsin-Madison Univ., Madison, WI (USA). Dept. of Horticulture), Davis, S.J

“…Green fluorescent protein (GFP) from Aequorea victoria has rapidly become a standard reporter in many biological systems. However, the use of GFP in higher…”
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High performance liquid chromatography resolution of ubiquitin pathway enzymes from wheat germ by Sullivan, Michael L., Callis, Judy, Vierstra, Richard D.

“…The highly conserved protein ubiquitin is involved in several cellular processes in eukaryotes as a result of its covalent ligation to a variety of target…”
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