Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Purpose To investigate the effect of experimental traumatic occlusion (ETO) induced by metal crowns on alveolar bone loss. Materials and Methods Metal crowns were custom‐made for the lower first molars with occlusal discrepancy of 0.4 and 0.7 mm from the maximum intercuspation. Thirty‐six animals we...

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Published in:Journal of prosthodontics Vol. 30; no. 2; pp. 142 - 149
Main Authors: Abdalla, Henrique Ballassini, Clemente‐Napimoga, Juliana Trindade, Trindade‐da‐Silva, Carlos Antônio, Alves, Luciane Jorge, Prats, Roberta da Silva, Youssef, Alexandre, Vieira dos Santos, Paulo César, Buarque e Silva, Wilkens Aurélio, Andrade e Silva, Frederico, Napimoga, Marcelo Henrique
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-02-2021
Subjects:
Alveolar bone
Alveolar Bone Loss - etiology
Animals
Bone loss
bone loss, inflammation
Bone resorption
Cementation
Crowns
Dentistry
Enzyme-linked immunosorbent assay
Enzymes
Lymphocytes T
Methylene blue
Molars
Occlusion
Osteoclasts
periodontal ligament
Periodontium
premature contact
TRANCE protein
Trauma
Traumatic occlusion
Tumor necrosis factor
Western blotting
bone loss, inflammation
periodontal ligament
premature contact
Traumatic occlusion
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Summary:Purpose To investigate the effect of experimental traumatic occlusion (ETO) induced by metal crowns on alveolar bone loss. Materials and Methods Metal crowns were custom‐made for the lower first molars with occlusal discrepancy of 0.4 and 0.7 mm from the maximum intercuspation. Thirty‐six animals were randomly divided into three groups (n = 12 animals per group): 0.4‐mm hyperocclusion group, 0.7‐mm hyperocclusion group and the sham group (no metal crown). Twenty‐eight days after crown cementation, the animals were euthanized and gingival tissue was collected to assess cytokine levels of IL‐17, IL‐6, and TNF‐α using enzyme‐linked immunosorbent assay (ELISA). Mandibles were stained with 1% methylene blue and alveolar bone levels were quantified. Western blotting was used to quantify the expression of receptor activator of nuclear factor κ B (RANK), and its ligand (RANKL), secreted osteoclastogenic factor of activated T cells (SOFAT) and TNF‐α‐converting enzyme (TACE). Also, mandibles were histologically processed and stained with hematoxylin and eosin, from which the presence of osteoclast‐like cells, multinucleated cells containing ≥3 nuclei was counted at 100× magnification. The data were analyzed using one‐way ANOVA and Tukey tests. Results Experimental occlusal trauma for 28 consecutive days significantly increased alveolar bone loss and multinucleated cell counts (p < 0.05). RANK, RANKL, SOFAT, TACE, IL‐6, and TNF‐α were significantly higher in gingival tissues of ETO groups (p < 0.05). IL‐17 titers were unchanged among the groups (p > 0.05). Conclusion Experimental traumatic occlusion activates and sustains bone resorption pathways in the periodontium inducing alveolar bone resorption. As the intensity of occlusal trauma increased, alternative osteoclastic pathways were activated, such as TACE and SOFAT.
Bibliography:This work was supported by São Paulo Research Foundation, FAPESP (#2017/22334‐9), Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Research Productivity Fellowships were awarded to MHN and JTCN) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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The authors explicitly state that they have no conflicts of interest in connection with this article
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ISSN:1059-941X
1532-849X
DOI:10.1111/jopr.13235