Oxidation reactions catalyzed by manganese peroxidase isoenzymes from Ceriporiopsis subvermispora

A total of 11 manganese peroxidase isoenzymes (MnP1-MnP11) with isoelectric points (pIs) in the range of 4.58–3.20 were isolated from liquid- and solid-state cultures of the basidiomycete Ceriporiopsis subvermispora. In the presence of hydrogen peroxide, these isoenzymes showed different requirement...

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Bibliographic Details
Published in:	FEBS letters Vol. 371; no. 2; pp. 132 - 136
Main Authors:	Urzúa, Ulises, Larrondo, Luis Fernando, Lobos, Sergio, Larraín, Juan, Vicun˜a, Rafael
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 04-09-1995
Subjects:	2,2-azino-di-(3-ethylbenz-thiazoline-6-sulfonate) 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one ABTS Basidiomycete Basidiomycota - enzymology Benzothiazoles Dianisidine - metabolism Electrophoresis, Polyacrylamide Gel Enzymatic oxidation Guaiacol - analogs & derivatives Guaiacol - metabolism IEF Isoelectric Point isoelectrofocusing Isoenzymes - chemistry Isoenzymes - isolation & purification Isoenzymes - metabolism kojic acid Lignin degradation Manganese Manganese - pharmacology manganese peroxidase MnP Oxalates - pharmacology Oxalic Acid Oxidation-Reduction Peroxidase Peroxidases - chemistry Peroxidases - isolation & purification Peroxidases - metabolism Pyrones - metabolism Sulfonic Acids - metabolism Basidiomycete kojic acid Lignin degradation Enzymatic oxidation pI Peroxidase IEF MnP ABTS Manganese
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Summary:	A total of 11 manganese peroxidase isoenzymes (MnP1-MnP11) with isoelectric points (pIs) in the range of 4.58–3.20 were isolated from liquid- and solid-state cultures of the basidiomycete Ceriporiopsis subvermispora. In the presence of hydrogen peroxide, these isoenzymes showed different requirements for Mn(II) in the oxidation of vanillylacetone, o-dianisidine, p-anisidine and ABTS, whereas oxidation of guaiacol by any isoenzyme did not take place when this metal was omitted. Km values for o-dianisidine and p-anisidine in the absence of Mn(II) are in the range of 0.5–1.0 mM and 4.5–42.0 mM, respectively. Oxalate and citrate, but not tartrate, accelerate the oxidation of o-dianisidine, both in the presence and in the absence of Mn(II). MnPs from this fungus are able to oxidize kojic acid without externally added hydrogen peroxide, indicating that they can also act as oxidases. In this reaction, however, the requirement for Mn(II) is absolute.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(95)00874-9