Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders

Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders

This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patien...

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Bibliographic Details
Published in:Cancers Vol. 16; no. 17; p. 2990
Main Authors: Rocchetti, Federica, Tenore, Gianluca, Macali, Federica, Vicidomini, Teresa, Podda, Gian Marco, Fantozzi, Paolo Junior, Silvestri, Valentina, Porzio, Virginia, Valentini, Virginia, Ottini, Laura, Richetta, Antonio Giovanni, Valentini, Valentino, Della Monaca, Marco, Grenga, Camilla, Polimeni, Antonella, Romeo, Umberto
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 28-08-2024
Subjects:
Alcohol
Biomarkers
Biopsy
Body fluids
Diagnosis
Disease management
Ethics
Health care
Medical prognosis
MicroRNAs
miRNA
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Patients
Plasma
Saliva
Squamous cell carcinoma
Statistical analysis
Tobacco
Variance analysis
Canada
United States > US
microRNAs
oral potentially malignant disorders
oral squamous cell carcinoma
liquid biopsy
non-invasive biomarkers
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Summary:This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients ( = 14); OPMDs patients ( = 6); and healthy controls ( = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups ( < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs.
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ISSN:2072-6694
2072-6694
DOI:10.3390/cancers16172990