Production of xanthosine-5'-monophosphate and inosine-5'-monophosphate by auxotrophic mutants of a coryneform bacterium

Although most microorganisms with genetic blocks in the purine nucleotide sequence excrete breakdown products, a coryneform bacterium was found to accumulate intact 5'-nucleotides in the extracellular medium. Adenineless mutants accumulated 0.4 to 0.6 g of inosine-5'-monophosphate per lite...

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Bibliographic Details
Published in:	Applied microbiology Vol. 13; no. 5; pp. 757 - 761
Main Authors:	Demain, A L, Jackson, M, Viatali, R A, Hendlin, D, Jacob, T A
Format:	Journal Article
Language:	English
Published:	United States 01-09-1965
Subjects:	Corynebacterium - metabolism In Vitro Techniques Micrococcus - metabolism Mutation Nucleotides - biosynthesis
Online Access:	Get full text
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Summary:	Although most microorganisms with genetic blocks in the purine nucleotide sequence excrete breakdown products, a coryneform bacterium was found to accumulate intact 5'-nucleotides in the extracellular medium. Adenineless mutants accumulated 0.4 to 0.6 g of inosine-5'-monophosphate per liter of broth. The yield of this nucleotide was increased to 0.8 to 0.9 g per liter when such mutants were mutated to xanthine dependence. Induction of a specific guanine requirement in adenineless auxotrophs resulted in cultures capable of producing high yields of xanthosine-5'-monophosphate (3 to 4 g per liter). Pure xanthosine-5'-monophosphate was isolated from broth by a procedure involving ion-exchange chromatography, charcoal adsorption, and barium precipitation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-6919
DOI:	10.1128/AEM.13.5.757-761.1965