RP-HPLC Method Development and Validation for the Simultaneous Estimation of Satranidazole and Ofloxacin in Pharmaceutical Dosage Form

RP-HPLC Method Development and Validation for the Simultaneous Estimation of Satranidazole and Ofloxacin in Pharmaceutical Dosage Form

A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of ofloxacin (OFL) and satranidazole (SAT) in combination. The separation is carried out using a mobile phase consist...

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Bibliographic Details
Published in:Journal of chromatographic science Vol. 49; no. 1; pp. 84 - 87
Main Authors: Bhoir, Suvarna I., Gaikwad, Poonam V., Parab, Lavu S., Shringarpure, Rohan N., Savant, Sudha S., Verma, Pratibha J.
Format: Journal Article
Language:English
Published: Niles, IL Oxford University Press 01-01-2011
Preston Publications
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Validation
Chemical analysis
HPLC chromatography
Oral form
Satranidazole
Ofloxacin
Fluoroquinolone derivatives
Reversed phase chromatography
Ultraviolet detector
Simultaneous measurement
Dosage form
Tablet
Antibacterial agent
Quinolone derivatives
Quantitative analysis
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Description
Summary:A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of ofloxacin (OFL) and satranidazole (SAT) in combination. The separation is carried out using a mobile phase consisting of 10mM phosphate buffer and methanol in the ratio of 50:50. The pH of the mobile phase is adjusted to 3.0 with 10% o-phosphoric acid. The column used is Kromasil-100 C18 (250 × 4.6 mm, 5 µm). with flow rate of 1.0 mL/min using UV detection at 294nm. The total run time is 5 min and the retention time of OFL and SAT is 2.59 min and 4.0 min respectively. The described method is linear for the assay of OFL and SAT over a concentration range of 10-24 µg/mL and 15-36 µg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The limit of quantitation for SAT and OFL has been found to be 0.042 µg/mL and 0.085 µg/mL respectively. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of SAT and OFL in bulk drug and its pharmaceutical dosage form.
ISSN:0021-9665
1945-239X
DOI:10.1093/chrsci/49.1.84