Electrophoretically Co-Deposited Collagen-Lactoferrin Membranes with Enhanced Pro-Regenerative Properties for Oral Soft Tissue Regeneration

Electrophoretically Co-Deposited Collagen-Lactoferrin Membranes with Enhanced Pro-Regenerative Properties for Oral Soft Tissue Regeneration

The quality of soft tissue defect regeneration after dental surgeries largely determines their final success. Collagen membranes have been proposed for the healing of such defects, but in some cases, they do not guarantee a sufficient volume of the regenerated tissue and vascularization. For this pu...

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 24; no. 24; p. 17330
Main Authors: Antoshin, Artem, Gostev, Mikhail, Khristidis, Yana, Giliazova, Aliia, Voloshin, Sergei, Blagushina, Nataliia, Smirnova, Olga, Diachkova, Ekaterina, Istranova, Elena, Usanova, Anna, Solodov, Nikolai, Fayzullin, Alexey, Ivanova, Elena, Sadchikova, Elena, Vergara Bashkatova, Milena Noelia, Drakina, Olga, Tarasenko, Svetlana, Timashev, Peter
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 10-12-2023
Subjects:
Biocompatibility
Biodegradation
Cell growth
Collagen
Connective tissue
Edema
electrophoretic deposition
Fibroblasts
free gingival graft harvesting
lactoferrin
Membranes
Metabolism
SBA-EPD
Tissue engineering
vestibuloplasty
SBA-EPD
vestibuloplasty
membrane
collagen
lactoferrin
electrophoretic deposition
free gingival graft harvesting
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Summary:The quality of soft tissue defect regeneration after dental surgeries largely determines their final success. Collagen membranes have been proposed for the healing of such defects, but in some cases, they do not guarantee a sufficient volume of the regenerated tissue and vascularization. For this purpose, lactoferrin, a protein with natural pro-regenerative, anti-inflammatory, and pro-angiogenic activity, can be added to collagen. In this article, we used a semipermeable barrier-assisted electrophoretic deposition (SBA-EPD) method for the production of collagen-lactoferrin membranes. The membrane structure was studied by SEM, and its mechanical properties were shown. The lactoferrin release kinetics were shown by ELISA within 75 h. When tested in vitro, we demonstrated that the collagen-lactoferrin membranes significantly increased the proliferation of keratinocytes (HaCaT) and fibroblasts (977hTERT) compared to blank collagen membranes. In vivo, on the vestibuloplasty and free gingival graft harvesting models, we showed that collagen-lactoferrin membranes decreased the wound inflammation and increased the healing rates and regeneration quality. In some parameters, collagen-lactoferrin membranes outperformed not only blank collagen membranes, but also the commercial membrane Mucograft . Thus, we proved that collagen-lactoferrin membranes produced by the SBA-EPD method may be a valuable alternative to commercially used membranes for soft tissue regeneration in the oral cavity.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242417330