Protein analysis by desorption electrospray ionization mass spectrometry and related methods

Protein analysis by desorption electrospray ionization mass spectrometry and related methods

As a tool for analyzing solid samples Desorption Electrospray Ionization MS (DESI‐MS) offers some very attractive features: samples require minimal treatment and can remain at atmosphere during the measurement, which have fueled the growth of the technique. In recent years there have been numerous a...

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Bibliographic Details
Published in:Journal of mass spectrometry. Vol. 48; no. 5; p. i
Main Authors: Douglass, K. A., Venter, A. R.
Format: Journal Article
Language:English
Published: Bognor Regis Blackwell Publishing Ltd 01-05-2013
Wiley Subscription Services, Inc
Subjects:
Desorption
Droplets
Ionization
Mass spectrometry
Proteins
Sprayers
Sprays
Online Access:Get full text
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Description
Summary:As a tool for analyzing solid samples Desorption Electrospray Ionization MS (DESI‐MS) offers some very attractive features: samples require minimal treatment and can remain at atmosphere during the measurement, which have fueled the growth of the technique. In recent years there have been numerous applications for using DESI to analyze a variety of compounds and surfaces which show DESI analysis of larger protein molecules can still present a challenge, particularly when high sensitivity is required. The droplet pickup model of ion formation has been proposed for protein desorption/ionization whereby the charged primary spray interacts with the sample surface to dissolve and desorb analyte molecules followed by desolvation and charge transfer. In the Special Feature, Professor Andre Venter and Kevin Douglass from Western Michigan University deconstruct the DESI process with a particular focus on large proteins and examine the efficiency of the desorption and ionization processes separately, in order to better understand of how each process contributes to the overall detection efficiency of large protein ions.
Bibliography:istex:8A6B8051C1E359FBE9AD439B96B1D89D153A60E3
ArticleID:JMS3188
ark:/67375/WNG-20LT7KSV-K
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.3188