Kisspeptin promotes follicular development through its effects on modulation of P450 aromatase expression and steroidogenesis in sheep ovarian follicles

Kisspeptin promotes follicular development through its effects on modulation of P450 aromatase expression and steroidogenesis in sheep ovarian follicles

The present study was conducted to ascertain whether the role of kisspeptin in promoting in vitro development of preantral follicles was through the regulation of P450 aromatase gene expression and steroidogenesis in sheep. Accordingly, the cumulus cells and oocytes were collected from different dev...

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Bibliographic Details
Published in:Reproduction in domestic animals Vol. 58; no. 9; pp. 1270 - 1278
Main Authors: Venkata Ratna, S, Prasada Rao, T, Pathipati, Deepa, Chaitanya Kumar, T V, Rambabu Naik, B, Siva Kumar, A V N
Format: Journal Article
Language:English
Published: Germany Blackwell Publishing Ltd 01-09-2023
Subjects:
17β-Estradiol
Aromatase
Cell culture
Culture media
Follicles
Gametocytes
Gene expression
Growth factors
Kiss1 protein
Maturation
Oocytes
Progesterone
Sex hormones
Steroidogenesis
Steroids
Synthesis
estradiol-17β
preantral follicle
mRNA
sheep
progesterone
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Summary:The present study was conducted to ascertain whether the role of kisspeptin in promoting in vitro development of preantral follicles was through the regulation of P450 aromatase gene expression and steroidogenesis in sheep. Accordingly, the cumulus cells and oocytes were collected from different development stages of preantral follicles grown in vivo and cultured in vitro in TCM199B (Group I), TCM199B + KP (10 μg/mL) (Group II) and Standard medium + KP (10 μg/mL). To measure the steroid (Estradiol-17β; E and Progesterone; P ) synthesis through ELISA, spent culture medium was collected separately from the same in vitro groups. E synthesis in the spent medium collected from all the three groups showed an increasing trend from PFs' exposed to respective culture media for 3 min to 2-day culture stage but decreased thereafter till 6-day culture stage. This is followed by a sharp increase in E concentration in the spent medium collected after in vitro maturation. However, P synthesis in group III followed increased pattern as the development progressed from PFs' exposed to culture medium for 3 min to in vitro maturation stage. The steroid production was observed at all stages of in vitro development in altered supplemented conditions. The steroid synthesis in the spent medium was highest in the 6 day cultured PFs' in Standard medium + KP matured in vitro for 24 h. Therefore, supplementation of kisspeptin along with other growth factors promoted steroid production in cultured preantral follicles far better than in other media.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.14430