Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant

Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant

Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 1...

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Bibliographic Details
Published in:International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 3; pp. 839 - 845
Main Authors: Tóth, E. M, Vengring, A, Homonnay, Z. G, Kéki, Zs, Spröer, C, Borsodi, A. K, Márialigeti, K, Schumann, P
Format: Journal Article
Language:English
Published: England International Union of Microbiological Societies 01-03-2014
Subjects:
Alphaproteobacteria - classification
Alphaproteobacteria - genetics
Alphaproteobacteria - isolation & purification
bacteria
Bacterial Typing Techniques
Base Composition
Bosea thiooxidans
Cell Wall - chemistry
cell walls
Diaminopimelic Acid - chemistry
DNA
DNA, Bacterial - genetics
fatty acid composition
fatty acids
Fatty Acids - chemistry
Hungary
Molecular Sequence Data
new genus
Nucleic Acid Hybridization
nucleotide sequences
phosphatidylcholines
phosphatidylethanolamines
Phospholipids - chemistry
Phylogeny
Power Plants
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
sequence homology
Ubiquinone - chemistry
Water Microbiology
Water Purification
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Summary:Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 %) and Bosea thiooxidans DSM 9653T (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18 : 1ω7c and 11-methyl C18 : 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol%. A new genus and species, Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T ( = DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.
Bibliography:http://dx.doi.org/10.1099/ijs.0.053843-0
ObjectType-Article-1
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ISSN:1466-5034
1466-5026
1466-5034
DOI:10.1099/ijs.0.053843-0