Isolation of Verocytotoxin-producing Escherichia coli O157 : H7 from cattle at slaughter in Italy

Cattle arriving for slaughter at a large abattoir in northern Italy between April 1997 and January 1998 were examined for intestinal carriage of Verocytotoxin-producing Escherichia coli (VTEC) O157 using an immunomagnetic separation technique. Sixty sorbitol non-fermenting VTEC O157 strains were iso...

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Bibliographic Details
Published in:	Veterinary microbiology Vol. 67; no. 3; pp. 203 - 211
Main Authors:	Bonardi, S., Maggi, E., Bottarelli, A., Pacciarini, M.L., Ansuini, A., Vellini, G., Morabito, S., Caprioli, A.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 30-06-1999 Elsevier Science
Subjects:	Abattoirs Animal productions Animals Bacterial Toxins - analysis Bacterial Toxins - biosynthesis Bacteriology Biological and medical sciences Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology Cattle-bacteria Disease Reservoirs DNA Primers - chemistry DNA, Viral - chemistry Escherichia coli Escherichia coli Infections - epidemiology Escherichia coli Infections - veterinary Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Feces - microbiology Female Fundamental and applied biological sciences. Psychology Glucuronidase - biosynthesis Immunomagnetic Separation - veterinary IMS Intestinal Diseases - epidemiology Intestinal Diseases - microbiology Intestinal Diseases - veterinary Italy Italy - epidemiology Latex Fixation Tests - veterinary Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase Chain Reaction - veterinary Prevalence Seasons Shiga Toxin 1 Terrestrial animal productions Urease - biosynthesis Vertebrates Italy Europe Cattle-bacteria Italy Escherichia coli IMS Prevalence Bovine Asymptomatic Shiga-like toxin Epidemiology Characterization Toxin Vertebrata Mammalia Seasonal variation Bacteria Isolation Artiodactyla Carrier Veterinary Ungulata Enterobacteriaceae
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Summary:	Cattle arriving for slaughter at a large abattoir in northern Italy between April 1997 and January 1998 were examined for intestinal carriage of Verocytotoxin-producing Escherichia coli (VTEC) O157 using an immunomagnetic separation technique. Sixty sorbitol non-fermenting VTEC O157 strains were isolated from 59 (13.1%) of the 450 cattle examined. In particular, VTEC O157 was found in 37 (16.6%) of 223 feedlot cattle and in 22 (16.1%) of 137 dairy cull cows, but not in the 90 veal calves sampled. The isolation rate was higher during warm weather (17.5%), falling to an average of 2.9% during the winter months. VT-negative, O157 latex-agglutinating E. coli strains were isolated from 23 (5.1%) of the 450 animals. PCR analysis showed that all 60 VTEC O157 strains carried the VT2 gene and that 25 strains also carried the VT1 gene. In addition, four of the VT-negative, O157 latex-agglutinating E. coli strains carried the VT2 gene. Atypical biochemical features were observed in some VTEC O157: two strains (3.3%) showed β-glucuronidase activity, and seven (11.7%) produced urease.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0378-1135 1873-2542
DOI:	10.1016/S0378-1135(99)00039-5