Search Results - "Velikodvorskaya, G. A."

Bacterial acetone and butanol production by industrial fermentation in the Soviet Union : use of hydrolyzed agricultural waste for biorefinery by ZVERLOV, V. V, BEREZINA, O, VELIKODVORSKAYA, G. A, SCHWARZ, W. H

“…Clostridial acetone-butanol fermentation from renewable carbohydrates used to be the largest biotechnological process second only to yeast ethanol fermentation…”
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Chimeric Lactase Capable of Spontaneous and Strong Immobilization on Cellulose and Development of a Continuous-Flow System for Lactose Hydrolysis at High Temperatures by Velikodvorskaya, G.A, Tikhonova, T.V, Gurvits, I.D, Karyagina, A.S, Lavrova, N.V, Sergienko, O.V, Tashlitskii, V.N, Lunina, N.A, Lunin, V.G

“…Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the…”
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Glucose-1-phosphatase (AgpE) from Enterobacter cloacae displays enhanced phytase activity by Herter, T, Berezina, O. V, Zinin, N. V, Velikodvorskaya, G. A, Greiner, R, Borriss, R

“…Using a screening procedure developed for detection of phytate hydrolysing enzymes, the gene agpE encoding glucose-1-phosphatase was cloned from an…”
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Properties of four C-terminal carbohydrate-binding modules (CBM4) of laminarinase Lic16A of Clostridium thermocellum by Dvortsov, I. A., Lunina, N. A., Zverlov, V. V., Velikodvorskaya, G. A.

“…At the C-terminus of multimodular laminarinase Lic16A from Clostridium thermocellum , four carbohydrate-binding modules (CBM) of family 4 were found. Isolated…”
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Substrate-binding properties of the family 54 module of Clostridium thermocellum Lic16A laminarinase by Dvortsov, I. A, Lunina, N. A, Zverlov, V. V, Velikodvorskaya, G. A

“…Endo-β-1.3-1.4-glucanase Lic16A of the moderate thermophilic anaerobe Clostridium thermocellum has a complex multimodular structure. In addition to the…”
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Ethidium bromide is good not only for staining of nucleic acids but also for staining of proteins after polyacrylamide gel soaking in trichloroacetic acid solution by Dvortsov, I.A., Lunina, N.A., Chekanovskaya, L.A., Shedova, E.N., Gening, L.V., Velikodvorskaya, G.A.

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Prospects for the Practical Application of Substrate-Binding Modules of Glycosyl Hydrolases by Volkov, I. Yu, Lunina, N. A, Velikodvorskaya, G. A

“…The properties of substrate-binding modules of glycosyl hydrolases have been reviewed. The variation of the properties of these modules makes them promising as…”
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Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5 by Zverlov, V.V, Velikodvorskaya, G.A, Schwarz, W.H, Kellermann, J, Staudenbauer, W.L

“…The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously…”
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Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation by Dakhova, O N, Kurepina, N E, Zverlov, V V, Svetlichnyi, V A, Velikodvorskaya, G A

“…A genomic library of thermophilic anaerobic eubacterium Thermatoga neapolitana was constructed in E. coli using the pTZ19R plasmid vector. Some groups of…”
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Extracellular glycosyl hydrolase activity of the Clostridium strains producing acetone, butanol, and ethanol by Berezina, O. V, Sineoky, S. P, Velikodvorskaya, G. A, Schwarz, W, Zverlov, V. V

“…Production of acetone, butanol, ethanol, acetic acid, and butyric acid by three strains of anaerobic bacteria, which we identified as Clostridium…”
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Extracellular glycosyl hydrolase activity of the Clostridium strains producing acetone, butanol, and ethanol by Berezina, O V, Sineoky, S P, Velikodvorskaya, G A, Schwarz, W, Zverlov, V V

“…Production of acetone, butanol, ethanol, acetic acid, and butyric acid by three strains of anaerobic bacteria, which we identified as Clostridium…”
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Cloning and expression of Clostridium thermocellum genes coding for thermostable exoglucanases (cellobiohydrolases) in Escherichia coli cells by Tuka, K, Zverlov, V V, Bumazkin, B K, Velikodvorskaya, G A, Strongin AYa

“…By special screening approach two independent Cl. thermocellum genes directing the synthesis of thermostable glucanases with an exo-mode of action have been…”
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Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-β-1, 3-glucanase bound to the outer cell surface by FUCHS, Klaus-Peter, ZVERLOV, Vladimir V, VELIKODVORSKAYA, Galina A, LOTTSPEICH, Friedrich, SCHWARZ, Wolfgang H

“…Clostridium thermocellum produces one major beta-1,3-glucanase. Genomic DNA fragments containing the gene were cloned from two strains, DSM1237(T) (6848 bp)…”
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A Cluster of Thermotoga neapolitana Genes Involved in the Degradation of Starch and Maltodextrins: the Molecular Structure of the Locus by Berezina, O V, Lunina, N A, Zverlov, V V, Naumoff, D G, Liebl, W, Velikodvorskaya, G A

“…A 5451-bp genome fragment of the hyperthermophilic anaerobic eubacterium Thermotoga neapolitana has been cloned and sequenced. The fragment contains one…”
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A Cluster of Thermotoga neapolitana Genes Involved in the Degradation of Starch and Maltodextrins: the Expression of the aglB and aglA Genes in E. coli and the Properties of the Recombinant Enzymes by Lunina, N A, Berezina, O V, Veith, B, Zverlov, V V, Vorobjeva, I P, Chekanovskaya, L A, Khromov, I S, Raasch, C, Liebl, W, Velikodvorskaya, G A

“…The aglB and aglA genes from the starch/maltodextrin utilization gene cluster of Thermotoga neapolitana were subcloned into pQE vectors for expression in…”
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Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli by Bumazkin, B K, Velikodvorskaya, G A, Tuka, K, Mogutov, M A, Strongin AYa

“…Six independent and distinct cel genes coding endoglucanases have been selected from C. thermocellum pUC19-based gene bank in E. coli TG1. E. coli-derived…”
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A newly described cellulosomal cellobiohydrolase, CelO, from Clostridium thermocellum: investigation of the exo-mode of hydrolysis, and binding capacity to crystalline cellulose by Zverlov, Vladimir V, Velikodvorskaya, Galina A, Schwarz, Wolfgang H

“…Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Sq., 123182 Moscow, Russia 1 Research Group Microbial Biotechnology, Technische…”
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Cloning and characterisation of a large metagenomic DNA fragment containing glycosyl-hydrolase genes by Shedova, E. N., Berezina, O. V., Lunina, N. A., Zverlov, V. V., Schwarz, W. H., Velikodvorskaya, G. A.

“…The problem of searching for and characterizing enzymes produced by uncultured microorganisms is presently settled by creating metagenomic libraries. A…”
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Expression of the Genes celA and xylA isolated from a fragment of metagenomic DNA in Escherichia coli by Shedova, E. N., Lunina, N. A., Berezina, O. V., Zverlov, V. V., Schwartz, V., Velikodvorskaya, G. A.

“…The glycosyl hydrolase genes cel5A and xyl3A previously isolated by us within a fragment of DNA from the methagenomic library of cow rumen microflora DNA were…”
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Purification and cellulosomal localization of Clostridium thermocellum mixed linkage β-glucanase LicB (1,3-1,4-β-D-glucanase) by ZVERLOV, V. V, FUCHS, K. P, SCHWARZ, W. H, VELIKOVORSKAYA, G. A

“…Clostridium thermocellum recombinant beta -(1,3-1,4)-glucanase LicB was expressed in Escherichia coli K-12 and purified to electrophoretic homogeneity…”
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