Search Results - "Veld, Pim J. Huis in ’t"

Characterization of a DNA exit gate in the human cohesin ring by in 't Veld, Pim J. Huis, Herzog, Franz, Ladurner, Rene, Davidson, Iain F., Piric, Sabina, Kreidl, Emanuel, Bhaskara, Venugopal, Aebersold, Ruedi, Peters, Jan-Michael

“…Chromosome segregation depends on sister chromatid cohesion mediated by cohesin. The cohesin subunits Smc1, Smc3, and Scc1 form tripartite rings that are…”
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Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling by Huis In 't Veld, Pim J, Volkov, Vladimir A, Stender, Isabelle D, Musacchio, Andrea, Dogterom, Marileen

“…Errorless chromosome segregation requires load-bearing attachments of the plus ends of spindle microtubules to chromosome structures named kinetochores. How…”
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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes by Weissmann, Florian, Petzold, Georg, VanderLinden, Ryan, Veld, Pim J. Huis in ’t, Brown, Nicholas G., Lampert, Fabienne, Westermann, Stefan, Stark, Holger, Schulman, Brenda A., Peters, Jan-Michael

“…Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene…”
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Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint by Allan, Lindsey A, Camacho Reis, Magda, Ciossani, Giuseppe, Huis in ‘t Veld, Pim J, Wohlgemuth, Sabine, Kops, Geert JPL, Musacchio, Andrea, Saurin, Adrian T

“…Cyclin B:CDK1 is the master kinase regulator of mitosis. We show here that, in addition to its kinase functions, mammalian Cyclin B also scaffolds a localised…”
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Reconstitution and use of highly active human CDK1:Cyclin‐B:CKS1 complexes by Huis in 't Veld, Pim J., Wohlgemuth, Sabine, Koerner, Carolin, Müller, Franziska, Janning, Petra, Musacchio, Andrea

“…As dividing cells transition into mitosis, hundreds of proteins are phosphorylated by a complex of cyclin‐dependent kinase 1 (CDK1) and Cyclin‐B, often at…”
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Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin by Nishiyama, Tomoko, Sykora, Martina M., Pim J. Huis in 't Veld, Mechtler, Karl, Peters, Jan-Michael

“…Sister chromatid cohesion depends on Sororin, a protein that stabilizes acetylated cohesin complexes on DNA by antagonizing the cohesin release factor…”
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The structural flexibility of MAD1 facilitates the assembly of the Mitotic Checkpoint Complex by Chen, Chu, Piano, Valentina, Alex, Amal, Han, Simon J. Y., Huis in ’t Veld, Pim J., Roy, Babhrubahan, Fergle, Daniel, Musacchio, Andrea, Joglekar, Ajit P.

“…The spindle assembly checkpoint (SAC) safeguards the genome during cell division by generating an effector molecule known as the Mitotic Checkpoint Complex…”
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Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces by Volkov, Vladimir A, Huis In 't Veld, Pim J, Dogterom, Marileen, Musacchio, Andrea

“…Presence of multiple copies of the microtubule-binding NDC80 complex is an evolutionary conserved feature of kinetochores, points of attachment of chromosomes…”
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Flexible Long-Range Loops in the VH Gene Region of the Igh Locus Facilitate the Generation of a Diverse Antibody Repertoire by Medvedovic, Jasna, Ebert, Anja, Tagoh, Hiromi, Tamir, Ido M., Schwickert, Tanja A., Novatchkova, Maria, Sun, Qiong, Huis in ‘t Veld, Pim J., Guo, Chunguang, Yoon, Hye Suk, Denizot, Yves, Holwerda, Sjoerd J.B., de Laat, Wouter, Cogné, Michel, Shi, Yang, Alt, Frederick W., Busslinger, Meinrad

“…The immunoglobulin heavy-chain (Igh) locus undergoes large-scale contraction in pro-B cells, which facilitates VH-DJH recombination by juxtaposing distal VH…”
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Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin by Friese, Alexandra, Faesen, Alex C., Huis in ‘t Veld, Pim J., Fischböck, Josef, Prumbaum, Daniel, Petrovic, Arsen, Raunser, Stefan, Herzog, Franz, Musacchio, Andrea

“…Accurate chromosome segregation during cell division is crucial for propagating life and protects from cellular transformation. The SKAP:Astrin heterodimer…”
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Rapid movement and transcriptional re-localization of human cohesin on DNA by Davidson, Iain F, Goetz, Daniela, Zaczek, Maciej P, Molodtsov, Maxim I, Huis in 't Veld, Pim J, Weissmann, Florian, Litos, Gabriele, Cisneros, David A, Ocampo-Hafalla, Maria, Ladurner, Rene, Uhlmann, Frank, Vaziri, Alipasha, Peters, Jan-Michael

“…The spatial organization, correct expression, repair, and segregation of eukaryotic genomes depend on cohesin, ring‐shaped protein complexes that are thought…”
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The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases by Ciossani, Giuseppe, Overlack, Katharina, Petrovic, Arsen, Huis in 't Veld, Pim J., Koerner, Carolin, Wohlgemuth, Sabine, Maffini, Stefano, Musacchio, Andrea

“…The segregation of chromosomes during cell division relies on the function of the kinetochores, protein complexes that physically connect chromosomes with…”
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Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding by Polley, Soumitra, Müschenborn, Helen, Terbeck, Melina, De Antoni, Anna, Vetter, Ingrid R, Dogterom, Marileen, Musacchio, Andrea, Volkov, Vladimir A, Huis in 't Veld, Pim J

“…During cell division, kinetochores link chromosomes to spindle microtubules. The Ndc80 complex, a crucial microtubule binder, populates each kinetochore with…”
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Structural basis of mRNA binding by the human FERRY Rab5 effector complex by Quentin, Dennis, Schuhmacher, Jan S., Klink, Björn U., Lauer, Jeni, Shaikh, Tanvir R., Huis in ’t Veld, Pim J., Welp, Luisa M., Urlaub, Henning, Zerial, Marino, Raunser, Stefan

“…The pentameric FERRY Rab5 effector complex is a molecular link between mRNA and early endosomes in mRNA intracellular distribution. Here, we determine the…”
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Molecular basis of outer kinetochore assembly on CENP-T by Huis In 't Veld, Pim J, Jeganathan, Sadasivam, Petrovic, Arsen, Singh, Priyanka, John, Juliane, Krenn, Veronica, Weissmann, Florian, Bange, Tanja, Musacchio, Andrea

“…Stable kinetochore-microtubule attachment is essential for cell division. It requires recruitment of outer kinetochore microtubule binders by centromere…”
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Cohesin’s ATPase Activity Couples Cohesin Loading onto DNA with Smc3 Acetylation by Ladurner, Rene, Bhaskara, Venugopal, Huis in ’t Veld, Pim J., Davidson, Iain F., Kreidl, Emanuel, Petzold, Georg, Peters, Jan-Michael

“…Cohesin mediates sister chromatid cohesion by topologically entrapping sister DNA molecules inside its ring structure. Cohesin is loaded onto DNA by the…”
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Topology and structure of an engineered human cohesin complex bound to Pds5B by Hons, Michael T., Huis in ‘t Veld, Pim J., Kaesler, Jan, Rombaut, Pascaline, Schleiffer, Alexander, Herzog, Franz, Stark, Holger, Peters, Jan-Michael

“…The cohesin subunits Smc1, Smc3 and Scc1 form large tripartite rings which mediate sister chromatid cohesion and chromatin structure. These are thought to…”
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FIRRM/C1orf112 is synthetic lethal with PICH and mediates RAD51 dynamics by Stok, Colin, Tsaridou, Stavroula, van den Tempel, Nathalie, Everts, Marieke, Wierenga, Elles, Bakker, Femke J., Kok, Yannick, Alves, Inês Teles, Jae, Lucas T., Raas, Maximilian W.D., Huis in 't Veld, Pim J., de Boer, H. Rudolf, Bhattacharya, Arkajyoti, Karanika, Eleftheria, Warner, Harry, Chen, Mengting, van de Kooij, Bert, Dessapt, Julien, ter Morsche, Lars, Perepelkina, Polina, Fradet-Turcotte, Amelie, Guryev, Victor, Tromer, Eelco C., Chan, Kok-Lung, Fehrmann, Rudolf S.N., van Vugt, Marcel A.T.M.

“…Joint DNA molecules are natural byproducts of DNA replication and repair. Persistent joint molecules give rise to ultrafine DNA bridges (UFBs) in mitosis,…”
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CDC20 assists its catalytic incorporation in the mitotic checkpoint complex by Piano, Valentina, Alex, Amal, Stege, Patricia, Maffini, Stefano, Stoppiello, Gerardo A, Huis In 't Veld, Pim J, Vetter, Ingrid R, Musacchio, Andrea

“…Open (O) and closed (C) topologies of HORMA-domain proteins are respectively associated with inactive and active states of fundamental cellular pathways. The…”
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Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis by Alao, John P., Huis in 't Veld, Pim J., Buhse, Frederike, Sunnerhagen, Per

“…Summary The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways…”
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