Role of connexin-43 hemichannels in the pathogenesis of Yersinia enterocolitica

Connexin (Cx) channels are sites of cytoplasmic communication between contacting cells. Evidence indicates that the opening of hemichannels occurs under both physiological and pathological conditions. In this paper, the involvement of Cx-43 hemichannels is demonstrated in the pathogenesis of Yersini...

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Published in:	The veterinary journal (1997) Vol. 182; no. 3; pp. 452 - 457
Main Authors:	Velasquez Almonacid, L.A., Tafuri, S., Dipineto, L., Matteoli, G., Fiorillo, E., Morte, R. Della, Fioretti, A., Menna, L.F., Staiano, N.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-12-2009
Subjects:	18-alpha-glycyrrhetinic acid Animals Bacterial Adhesion bacterial uptake cell communication Colony Count, Microbial Connexin 43 - antagonists & inhibitors Connexin 43 - physiology Connexin-43 cytoplasm Glycyrrhetinic Acid - analogs & derivatives Glycyrrhetinic Acid - pharmacology HeLa cell HeLa Cells human cell lines Humans in vitro studies Ion Channel Gating - physiology Isoquinolines - metabolism pathogenesis permeability Phosphorylation protein phosphorylation Signalling tyrosine Tyrosine - metabolism tyrosine phosphorylation Uptake Western blotting Yersinia enterocolitica Yersinia enterocolitica - pathogenicity yersiniosis Yersinia enterocolitica Connexin-43 Signalling Uptake HeLa cell
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Summary:	Connexin (Cx) channels are sites of cytoplasmic communication between contacting cells. Evidence indicates that the opening of hemichannels occurs under both physiological and pathological conditions. In this paper, the involvement of Cx-43 hemichannels is demonstrated in the pathogenesis of Yersinia. Parental HeLa cells and transfected HeLa cells stably expressing Cx-43 (HCx43) were infected with Yersinia enterocolitica, and bacterial uptake was measured by the colony-forming unit method. Bacterial uptake was higher in HCx43 cells than in parental cells and was inhibited by the Cx channel blocker, 18-α-glycyrrhetinic acid (AGA). The inhibitory effect of AGA was more pronounced on the Y. enterocolitica uptake by HCx43 cells than by parental cells. The ability of HCx43 cells to incorporate the permeable fluorescent tracer Lucifer Yellow (LY) was assessed. Dye incorporation was inhibited by AGA, whereas Y. enterocolitica infection of HCx43 cells increased LY incorporation. Western blotting analysis demonstrated that Y. enterocolitica infection of HCx43 cells induced tyrosine phosphorylation of Cx-43, thus supporting a critical role for Cx-43 in the strategies exploited by bacterial pathogens to invade non-phagocytic cells.
Bibliography:	http://dx.doi.org/10.1016/j.tvjl.2008.08.011 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1090-0233 1532-2971
DOI:	10.1016/j.tvjl.2008.08.011