Search Results - "Velasco, P. T."

Why Alzheimer's is a disease of memory: the attack on synapses by A beta oligomers (ADDLs) by Viola, K L, Velasco, P T, Klein, W L

“…Individuals with early-stage Alzheimer's disease (AD) suffer from profound failure to form new memories. A novel molecular mechanism with implications for…”
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Isolation of Transglutaminase-Reactive Sequences from Complex Biological Systems: A Prominent Lysine Donor Sequence in Bovine Lens by Lorand, L., Valasco, P. T., Murthy, S. N. P., Wilson, J., Parameswaran, K. N.

“…The transglutaminase (protein-glutamine: amine γ-glutamyltransferase, EC 2.3.2.13)-catalyzed cross-linking of proteins in biological systems can often be…”
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Labeling of ε-Lysine Crosslinking Sites in Proteins with Peptide Substrates of Factor XIIIa and Transglutaminase by Parameswaran, K. N., Velasco, P. T., Wilson, J., Lorand, L.

“…Peptides patterned on the N-terminal sequence of fibronectin were synthesized and tested for amine acceptor qualities in reactions with dansylcadaverine…”
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Sorting-Out of Acceptor-Donor Relationships in the Transglutaminase- Catalyzed Cross-Linking of Crystallins by the Enzyme-Directed Labeling of Potential Sites by Lorand, L., Parameswaran, K. N., Velasco, P. T.

“…The dansyl-conjugated (Dns) peptides Dns-Pro-Gly-Gly-Gln-Gln-Ile-Val and Dns-Ala-Gln-Gln-Ile-Val, patterned on the N-terminal sequence of fibronectin, were…”
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Novel Inhibitors Against the Transglutaminase-catalysed Crosslinking of Lens Proteins by LORAND, LASZLO, STERN, ANDREW M., VELASCO, PAULINE T.

“…Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and…”
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Properties of Purified Lens Transglutaminase and Regulation of its Transamidase/Crosslinking Activity by GTP by PRASANNA MURTHY, S.N., VELASCO, PAULINE T., LORAND, LASZLO

“…On account of its protein crosslinking activity, the Ca2+-dependent transglutaminase of the lens is likely to be involved in the formation of cataracts. We…”
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Association of a Transglutaminase-Related Antigen with Intermediate Filaments by Trejo-Skalli, Amy V., Velasco, Pauline T., S. N. Prasanna Murthy, Lorand, Laszlo, Goldman, Robert D.

“…A mouse monoclonal antibody, G92.1.2, raised against guinea pig liver transglutaminase (TGase) recognizes an antigen present in primary mouse dermal…”
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Hierarchy of Lens Proteins Requiring Protection Against Heat-Induced Precipitation by the α Crystallin Chaperone by VELASCO, PAULINE T., LUKAS, THOMAS J., MURTHY, S.N.PRASANNA, DUGLAS-TABOR, YVONNE, GARLAND, DONITA L., LORAND, LASZLO

“…Gel filtration of the water-soluble extract from bovine lens yields a group of proteins, emerging between the peaks of βHand βLcrystallins, which show a…”
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A Transglutaminase-Related Antigen Associates with Keratin Filaments in Some Mouse Epidermal Cells by Clement, Sophie, Trejo-Skalli, Amy V., Gu, Lin, Velasco, Pauline T., Lorand, Laszlo, Goldman, Robert D.

“…A mouse monoclonal IgG, G82, directed against guinea pig liver transglutaminase recognizes a transglutaminase-related antigen that is associated with the…”
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Autoimmune Antibody (IgG Kansas) against the Fibrin Stabilizing Factor (Factor XIII) System by Lorand, L., Velasco, P. T., Rinne, J. R., Amare, M., Miller, L. K., Zucker, M. L.

“…Serum from a patient who died from massive hemorrhage within 4 months after onset of an acquired bleeding disorder at age 85 contained a potent inhibitor of…”
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Acceptor-donor relationships in the transglutaminase-mediated cross-linking of lens .beta.-crystallin subunits by Velasco, P. T, Lorand, L

“…Following the isolation of the N epsilon-(gamma-glutamyl)lysine-containing polymers from human cataracts, our efforts were directed to induce such cross-links…”
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Biotinylated peptides containing a factor XIIIa or a tissue transglutaminase-reactive glutaminyl residue that block protein cross-linking phenomena by becoming incorporated into amine donor sites by Lorand, L., Parameswaran, K. N., Velasco, P. T., Murthy, S. N. P.

“…Biotinylated peptides Biot-Gln-Gln-Ile-Val and Biot-epsilon-Aca-Gln-Gln-Ile-Val were shown to act as acceptor substrates for amines in reactions catalyzed by…”
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Probing the transglutaminase-mediated, posttranslational modification of proteins during development by Cariello, L, Velasco, P. T, Wilson, J, Parameswaran, K. N, Karush, F, Lorand, L

“…Sphaerechinus granularis eggs were fertilized in seawater in the presence of 0.2 mM dansylcadaverine, and development was allowed to take place with this…”
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Why Alzheimer’s is a disease of memory: The attack on synapses by Aß oligomers (ADDLs) by Viola, K. L., Velasco, P. T., Klein, W. L.

“…Individuals with early-stage Alzheimer’s disease (AD) suffer from profound failure to form new memories. A novel molecular mechanism with implications for…”
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Amide bond cleavage monitored continuously through detection of a dansylcadaverine leaving group by Lorand, L, Murthy, S N, Parameswaran, K N, Velasco, P T, Wilson, J

“…The transglutaminase-catalyzed incorporation of the fluorescent amine, dansylcadaverine, into casein derivatives, such as N,N-dimethylcasein, is accompanied by…”
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Transamidating activities of factor XIIIa and of transglutaminases, measured by an ELISA procedure by Velasco, P T, Karush, F, Lorand, L

“…The dansyl hapten in dansylcadaverine offers unique possibilities for measuring the incorporation of the monoamine into proteins (e.g. N,N'-dimethylcasein) by…”
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Specificity of guinea pig liver transglutaminase for amine substrates by Lorand, L., Parameswaran, K. N., Stenberg, P., Tong, Y. S., Velasco, P. T., Jonsson, N. A., Mikiver, L., Moses, P.

“…The amine specificity of guinea pig liver transglutaminase, a model enzyme for endo-gamma-glutamine:epsilon-lysin transferases, was explored with the aid of…”
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Identification of transglutaminase substrates in inside-out vesicles from human erythrocytes: immunoblotting with anti-dansyl antibody by Lorand, L, Murthy, S N, Velasco, P T, Karush, F

“…An immunoblotting procedure, using anti-dansyl antibody, was employed to demonstrate that band 3 protein was the predominant substrate in inside-out vesicles…”
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The Intermediate Filament Protein, Vimentin, in the Lens Is a Target for Cross-linking by Transglutaminase by Clément, S, Velasco, P T, Murthy, S N, Wilson, J H, Lukas, T J, Goldman, R D, Lorand, L

“…Mere addition of Ca 2+ to a lens cortical homogenate (bovine) generates a series of products composed of a variety of high molecular weight vimentin species…”
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Inhibition of beta-crystallin cross-linking in the Ca2+-treated lens by Lorand, L, Conrad, SM, Velasco, PT

“…beta-Crystallin dimers of approximately 55,000 weight are among the early products of protein cross-linking in Ca2+-treated rabbit lens, caused by the…”
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