Search Results - "Vedantham, Ganesh"

A Holistic Approach for Protein Secondary Structure Estimation from Infrared Spectra in H2O Solutions by Vedantham, Ganesh, Sparks, H.Gerald, Sane, Samir U., Tzannis, Stelios, Przybycien, Todd M.

“…We present an improved technique for estimating protein secondary structure content from amide I and amide III band infrared spectra. This technique combines…”
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Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification by Brodsky, Yan, Zhang, Cheng, Yigzaw, Yinges, Vedantham, Ganesh

“…We report the use of caprylic acid based impurity precipitation as (1) an alternative method to polishing chromatography techniques commonly used for…”
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Modeling and robust pooling design of a preparative cation-exchange chromatography step for purification of monoclonal antibody monomer from aggregates by Borg, Niklas, Brodsky, Yan, Moscariello, John, Vunnum, Suresh, Vedantham, Ganesh, Westerberg, Karin, Nilsson, Bernt

“…•A general rate model with temperature dependent diffusion describes the transport.•A pH- and temperature-dependent steric mass action model describes the…”
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Protein unfolding at interfaces: Slow dynamics of α-helix to β-sheet transition by Sethuraman, Ananthakrishnan, Vedantham, Ganesh, Imoto, Taiji, Przybycien, Todd, Belfort, Georges

“…A two‐phase sequential dynamic change in the secondary structure of hen egg lysozyme (Lys) adsorbed on solid substrates was observed. The first phase involved…”
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Hydrophobic interaction chromatography in dual salt system increases protein binding capacity by Senczuk, Anna M, Klinke, Ralph, Arakawa, Tsutomu, Vedantham, Ganesh, Yigzaw, Yinges

“…Hydrophobic interaction chromatography (HIC) uses weakly hydrophobic resins and requires a salting-out salt to promote protein-resin interaction. The…”
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Multipronged approach to managing beta-glucan contaminants in the downstream process: Control of raw materials and filtration with charge-modified nylon 6,6 membrane filters by Gefroh, Eva, Hewig, Art, Vedantham, Ganesh, McClure, Megan, Krivosheyeva, Alla, Lajmi, Ajay, Lu, Yuefeng

“…(1→3)‐β‐d‐Glucans (beta‐glucans) have been found in raw materials used in the manufacture of recombinant therapeutics. Because of their biological activity,…”
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Use of MMV as a Single Worst-Case Model Virus in Viral Filter Validation Studies by Gefroh, Eva, Dehghani, Houman, McClure, Megan, Connell-Crowley, Lisa, Vedantham, Ganesh

“…Typical platform processes for biopharmaceutical products derived from animal cell lines include a parvovirus filtration unit operation to provide viral safety…”
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Cation exchange chromatography provides effective retrovirus clearance for antibody purification processes by Connell-Crowley, Lisa, Nguyen, Thao, Bach, Julia, Chinniah, Shivanthi, Bashiri, Houman, Gillespie, Ron, Moscariello, John, Hinckley, Peter, Dehghani, Houman, Vunnum, Suresh, Vedantham, Ganesh

“…One measure taken to ensure safety of biotherapeutics produced in mammalian cells is to demonstrate the clearance of potential viral contaminants by downstream…”
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The structural response of proteins to bioseparations and drug delivery environment by Vedantham, Ganesh

“…The physical stability of pharmaceutical proteins in processing, storage, and delivery environments is a critical determinant of success of these steps…”
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Self-Assembled Monolayers on Polymer Surfaces:  Kinetics, Functionalization, and Photopatterning by Böhme, Peter, Vedantham, Ganesh, Przybycien, Todd, Belfort, Georges

“…Monolayers of bifunctional bolaamphiphiles were self-assembled on a polymeric substrate (spin-coated film of poly(allylamine hydrochloride)). Various features…”
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The structural response of proteins to bioseparations and drug delivery environment by Vedantham, Ganesh

“…The physical stability of pharmaceutical proteins in processing, storage, and delivery environments is a critical determinant of success of these steps…”
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Structural Response of Bovine Growth Hormone to Dead-Ended Ultrafiltration by Vedantham, Ganesh, Carothers, Stacey L., Belfort, Georges, Przybycien, Todd M.

“…Bovine growth hormone (bGH) was used as a model pharmaceutical protein to assess protein structural stability during dynamic ultrafiltration. Two popular…”
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