Search Results - "Vecchio, Alex"

Claudin-9 structures reveal mechanism for toxin-induced gut barrier breakdown by Vecchio, Alex J., Stroud, Robert M.

“…The human pathogenic bacterium Clostridium perfringens secretes an enterotoxin (CpE) that targets claudins through its C-terminal receptor-binding domain…”
Get full text

Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry by Lucido, Michael J, Orlando, Benjamin J, Vecchio, Alex J, Malkowski, Michael G

“…Aspirin and other nonsteroidal anti-inflammatory drugs target the cyclooxygenase enzymes (COX-1 and COX-2) to block the formation of prostaglandins. Aspirin is…”
Get full text

Structural basis for Clostridium perfringens enterotoxin targeting of claudins at tight junctions in mammalian gut by Vecchio, Alex J., Rathnayake, Sewwandi S., Stroud, Robert M.

“…The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F…”
Get full text

Highlighting membrane protein structure and function: A celebration of the Protein Data Bank by Li, Fei, Egea, Pascal F., Vecchio, Alex J., Asial, Ignacio, Gupta, Meghna, Paulino, Joana, Bajaj, Ruchika, Dickinson, Miles Sasha, Ferguson-Miller, Shelagh, Monk, Brian C., Stroud, Robert M.

“…Biological membranes define the boundaries of cells and compartmentalize the chemical and physical processes required for life. Many biological processes are…”
Get full text

Structural Basis of Fatty Acid Substrate Binding to Cyclooxygenase-2 by Vecchio, Alex J., Simmons, Danielle M., Malkowski, Michael G.

“…The cyclooxygenases (COX-1 and COX-2) are membrane-associated heme-containing homodimers that generate prostaglandin H2 from arachidonic acid (AA). Although AA…”
Get full text

Claudin-1 interacts with EPHA2 to promote cancer stemness and chemoresistance in colorectal cancer by Primeaux, Mark, Liu, Xiangdong, Gowrikumar, Saiprasad, Fatima, Iram, Fisher, Kurt W., Bastola, Dhundy, Vecchio, Alex J., Singh, Amar B., Dhawan, Punita

“…Therapy resistance is the primary problem in treating late-stage colorectal cancer (CRC). Claudins are frequently dysregulated in cancer, and several are being…”
Get full text

Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin by Ogbu, Chinemerem P, Kapoor, Srajan, Vecchio, Alex J

“…Clostridium perfringens enterotoxin (CpE) is a β-pore forming toxin that disrupts gastrointestinal homeostasis in mammals by binding membrane protein receptors…”
Get full text

Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes by Orlando, Benjamin J., Dominik, Pawel K., Roy, Sourav, Ogbu, Chinemerem P., Erramilli, Satchal K., Kossiakoff, Anthony A., Vecchio, Alex J.

“…Strains of Clostridium perfringens produce a two-domain enterotoxin (CpE) that afflicts humans and domesticated animals, causing prevalent gastrointestinal…”
Get full text

Computational design of soluble and functional membrane protein analogues by Goverde, Casper A., Pacesa, Martin, Goldbach, Nicolas, Dornfeld, Lars J., Balbi, Petra E. M., Georgeon, Sandrine, Rosset, Stéphane, Kapoor, Srajan, Choudhury, Jagrity, Dauparas, Justas, Schellhaas, Christian, Kozlov, Simon, Baker, David, Ovchinnikov, Sergey, Vecchio, Alex J., Correia, Bruno E.

“…De novo design of complex protein folds using solely computational means remains a substantial challenge 1 . Here we use a robust deep learning pipeline to…”
Get full text

Disruption of Claudin-Made Tight Junction Barriers by Clostridium perfringens Enterotoxin: Insights from Structural Biology by Ogbu, Chinemerem P, Roy, Sourav, Vecchio, Alex J

“…Claudins are a family of integral membrane proteins that enable epithelial cell/cell interactions by localizing to and driving the formation of tight…”
Get full text

High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography by Vecchio, Alex J, Stroud, Robert M

“…Structural biology has revealed predicting heterologous expression levels, homogeneity, and stability of a protein from its primary structure are exceedingly…”
Get more information

Investigating substrate promiscuity in cyclooxygenase-2: the role of Arg-120 and residues lining the hydrophobic groove by Vecchio, Alex J, Orlando, Benjamin J, Nandagiri, Ritwik, Malkowski, Michael G

“…The cyclooxygenases (COX-1 and COX-2) generate prostaglandin H(2) from arachidonic acid (AA). In its catalytically productive conformation, AA binds within the…”
Get full text

The Structural Basis of Endocannabinoid Oxygenation by Cyclooxygenase-2 by Vecchio, Alex J., Malkowski, Michael G.

“…The cyclooxygenases (COX-1 and COX-2) oxygenate arachidonic acid (AA) in the committed step of prostaglandin biogenesis. Substitutions of I434V, H513R, and…”
Get full text

Human Cyclooxygenase-2 Is a Sequence Homodimer That Functions as a Conformational Heterodimer by Dong, Liang, Vecchio, Alex J., Sharma, Narayan P., Jurban, Brice J., Malkowski, Michael G., Smith, William L.

“…Prostaglandin endoperoxide H synthases 1 and 2, also known as cyclooxygenases (COXs) 1 and 2, convert arachidonic acid (AA) to prostaglandin endoperoxide H2…”
Get full text

Identification and characterization of a first-generation inhibitor of claudin-1 in colon cancer progression and metastasis by Fatima, Iram, Uppada, Jaya Prakash, Chhonker, Yashpal S., Gowrikumar, Saiprasad, Barman, Susmita, Roy, Sourav, Tolentino, Kirsten T., Palermo, Nicholas, Natarajan, Amar, Beauchamp, Daniel R., Vecchio, Alex, Murry, Daryl J., Singh, Amar B., Hopkins, Corey R., Dhawan, Punita

“…Colorectal cancer (CRC) is a leading cause of the cancer-related deaths worldwide. Thus, developing novel and targeted therapies for inhibiting CRC progression…”
Get full text

The structure of NS-398 bound to cyclooxygenase-2 by Vecchio, Alex J., Malkowski, Michael G.

“…The cyclooxygenases (COX-1 and COX-2) are membrane-associated, heme-containing homodimers that generate prostaglandin H 2 from arachidonic acid (AA) in the…”
Get full text

Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment by Erramilli, Satchal K., Dominik, Pawel K., Ogbu, Chinemerem P., Kossiakoff, Anthony A., Vecchio, Alex J.

“…Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between…”
Get full text

Molecular and Structural Basis Underlying Selective Targeting of Claudins by Clostridium perfringens Enterotoxin in Mammalian Gut by Vecchio, Alex, Rathnayake, Sewwandi, Stroud, Robert

“…The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F…”
Get full text

Structural basis for human claudin‐9 ion selectivity and tight junction dissociation by a bacterial toxin by Vecchio, Alex J, Stroud, Robert M

“…Tight junctions are macromolecular polymers involved in cell−cell adhesion and intercellular transport of solutes through epithelial or endothelial cell…”
Get full text

Structural Basis of IClostridium perfringens/I Enterotoxin Activation and Oligomerization by Trypsin by Ogbu, Chinemerem P, Kapoor, Srajan, Vecchio, Alex J

“…Clostridium perfringens enterotoxin (CpE) is a β-pore forming toxin that disrupts gastrointestinal homeostasis in mammals by binding membrane protein receptors…”
Get full text