NFκB Expression during Cold Ischemia Correlates with Postreperfusion Graft Function

NFκB Expression during Cold Ischemia Correlates with Postreperfusion Graft Function

In liver transplantation, activation of NFκB occurs upon reperfusion, yet few data exist regarding NFκB activation during cold ischemia. We hypothesized that activation of NFκB may initially occur during cold ischemia, prior to reperfusion, and serve as an important determinant of postreperfusion fu...

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Bibliographic Details
Published in:The Journal of surgical research Vol. 93; no. 1; pp. 35 - 40
Main Authors: Ricciardi, R., Kim, R.D., McDade, T.P., Perugini, R.A., Veal, T.M., Quarfordt, S.H., Callery, M.P., Chari, R.S., Meyers, W.C.
Format: Journal Article Conference Proceeding
Language:English
Published: New York, NY Elsevier Inc 01-09-2000
Elsevier
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Clinical death. Palliative care. Organ gift and preservation
function
ischemia
liver
Liver, biliary tract, pancreas, portal circulation, spleen
Medical sciences
NFκB
reperfusion
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the digestive system
NFκB
liver
reperfusion
function
ischemia
Liver
Cardiovascular disease
Transplantation
Gene expression
Pig
Liver function
Vertebrata
Organ donation
Mammalia
Reperfusion
Ischemia
Surgery
Animal
Artiodactyla
Transcription factor NFκB
Transcription factor
Ungulata
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Summary:In liver transplantation, activation of NFκB occurs upon reperfusion, yet few data exist regarding NFκB activation during cold ischemia. We hypothesized that activation of NFκB may initially occur during cold ischemia, prior to reperfusion, and serve as an important determinant of postreperfusion function. To test this hypothesis, serial biopsies during porcine liver harvest were obtained immediately upon laparotomy, upon completion of dissection, after 45 and 120 min of cold ischemia, and 60 and 180 min after reperfusion. Nuclear extracts were isolated for Western blot analysis of NFκB. Hepatic function was assessed through bile output and sorbitol dehydrogenase (SDH) activity. NFκB expression was maximal at 45 min of cold ischemia and decreased by 120 min. The expression at 120 min of cold ischemia correlated with markers of postreperfusion function, namely bile flow and SDH activity. During reperfusion a second distinct peak occurred at 180 min. Increased expression of NFκB at 180 min of reperfusion correlated directly with prior expression at 120 min during cold ischemia and with increased SDH activity. These data indicate that nuclear expression of NFκB demonstrate two distinct peaks of activity, one during cold ischemia and one after reperfusion. Enhanced expression of NFκB during cold ischemia not only correlates directly with NFκB expression during reperfusion, but also correlates inversely with postreperfusion graft function.
ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.2000.5961