Fatty Acid Desaturases in the chloroplast and endoplasmic reticulum promote susceptibility to the Green Peach Aphid, Myzus persicae , in Arabidopsis thaliana

Fatty Acid Desaturases in the chloroplast and endoplasmic reticulum promote susceptibility to the Green Peach Aphid, Myzus persicae , in Arabidopsis thaliana

Fatty acid desaturases (FADs) in plants influence levels of susceptibility to multiple stresses, including insect infestations. In this study, infestations of the greAtFABen peach aphid ( ) on were reduced by mutations in three desaturases: , which encodes a chloroplastic stearoyl-[acyl-carrier-prot...

Full description

Saved in:
Bibliographic Details
Published in:Molecular plant-microbe interactions Vol. 34; no. 6; pp. 691 - 702
Main Authors: Li, Jiamei, Galla, Aravind L, Avila, Carlos Augusto, Flattmann, Kaitlin, Vaughn, Kaleb L, Goggin, Fiona
Format: Journal Article
Language:English
Published: United States American Phytopathological Society 01-06-2021
The American Phytopathological Society
Subjects:
Aphidoidea
Arabidopsis thaliana
Chloroplasts
Desaturase
Endoplasmic reticulum
Fatty acids
Insects
Mutants
Mutation
Myzus persicae
Pest resistance
Population studies
Susceptibility
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fatty acid desaturases (FADs) in plants influence levels of susceptibility to multiple stresses, including insect infestations. In this study, infestations of the greAtFABen peach aphid ( ) on were reduced by mutations in three desaturases: , which encodes a chloroplastic stearoyl-[acyl-carrier-protein] 9-desaturase, and or , which encode ω-3 FADs in the chloroplast and endoplasmic reticulum (ER) respectively. These data indicate that certain FADs promote susceptibility to aphids, and that aphids are impacted by desaturases in both the chloroplast and ER. Aphid resistance in , , and/or might involve altered signaling between these subcellular compartments. C18:1 levels are depleted in , whereas C18:2 accumulation is enhanced in and . In contrast, has higher than normal C18:2 levels but also high C18:1 and low C18:0, and does not impact aphid numbers. Potentially, aphids may be influenced by the balance of multiple fatty acids (FAs) rather than by a single species, with C18:2 promoting aphid resistance and C18:1 promoting susceptibility. Although the mutant also accumulates higher-than-normal levels of C16:2, this FA does not contribute to aphid resistance because a triple mutant line that lacks detectable levels of C16:2 ( ) retains comparable levels of aphid resistance as fad7. In addition, aphid numbers are unaffected by the mutation that inhibits C16:1 synthesis. Together, these results demonstrate that certain FADs are important susceptibility factors in plant-aphid interactions, and that aphid resistance is more strongly associated with differences in C18 abundance than C16 abundance.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0894-0282
1943-7706
DOI:10.1094/MPMI-12-20-0345-R