Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation

The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10%...

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Bibliographic Details
Published in:	Brazilian dental journal Vol. 13; no. 3; pp. 162 - 165
Main Authors:	de Castro Albuquerque, Rodrigo, Gomez, Ricardo Santiago, Dutra, Rodrigo Aliprandi, Vasconcellos, Wallison Arthuso, Gomez, Renato Santiago, Gomez, Marcus Vinícius
Format:	Journal Article
Language:	English
Published:	Brazil Fundação Odontológica de Ribeirão Preto 2002
Subjects:	Animals carbamide peroxide carcinogenesis Cell Division - drug effects Drug Combinations Epithelial Cells - drug effects Epithelial Cells - metabolism immunocytochemistry Immunoenzyme Techniques Male Mouth Mucosa - drug effects Mouth Mucosa - metabolism oral mucosa Oxidants - toxicity Peroxides - toxicity proliferating cell nuclear antigen Proliferating Cell Nuclear Antigen - biosynthesis Rats Rats, Wistar Statistics, Nonparametric Tongue - cytology Tooth Bleaching - adverse effects Urea - analogs & derivatives Urea - toxicity
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Summary:	The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10% carbamide peroxide on one side of the dorsal tongue once a week for three consecutive weeks. Only distilled water was applied on the control side. The animals were killed on days 0, 10, and 20 after the last application. The tongue was fixed in buffered formalin for 24 h and embedded in paraffin. Tissue blocks (3 microns) were subjected to the biotin-streptavidin amplified system for identification of PCNA. The percentage of epithelial-positive basal cells in each side of the tongue mucosa was calculated. The results demonstrated that topical application of 10% carbamide peroxide increases PCNA immunohistochemical expression on the basal layer of the oral mucosa epithelium of rats on day 0 after treatment. In conclusion, short-course use of carbamide peroxide induces transient epithelial cell proliferation of the oral mucosa of rats.
ISSN:	0103-6440 0103-6440
DOI:	10.1590/S0103-64402002000300003