Elevated Hepatic Glucocorticoid Receptor Expression During Liver Regeneration in Rats

Elevated Hepatic Glucocorticoid Receptor Expression During Liver Regeneration in Rats

In rats within the first week of partial hepatectomy reconstruction of the normal histological structure of the liver already starts. To approach the possible role of endogenous glucocorticoids in the process of regeneration we measured the changes in the expression of steroid glucocorticoid recepto...

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Bibliographic Details
Published in:Pathology oncology research Vol. 5; no. 2; pp. 107 - 109
Main Authors: Karabélyos, Csaba, Dobozy, Ottó, Szalai, Csaba, Klenjánszki, Katalin, Varjú, Kornélia, Hadházi, Ákos, Kiss, Árpád, Fülöp, András Kristóf, Madarász, Bálint, Falus, András
Format: Journal Article
Language:English
Published: London Elsevier Inc 01-06-1999
Saunders
Springer Nature B.V
Subjects:
Animals
Biological and medical sciences
Female
Gene expression
Immunohistochemistry
Liver
Liver - metabolism
Liver - pathology
Liver Regeneration
Liver, biliary tract, pancreas, portal circulation, spleen
Male
Medical sciences
Oncology
Pathology
Proteins
Rats
Rats, Wistar
Receptors, Glucocorticoid - biosynthesis
Rodents
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the digestive system
Immunohistochemistry
Animal model
Steroid hormone
Liver
Gene expression
Glucocorticoid
Mechanism
Hepatectomy
Pathology
Regeneration
Partial
Surgery
Animal
Adrenal hormone
Molecular biology
Biological receptor
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Summary:In rats within the first week of partial hepatectomy reconstruction of the normal histological structure of the liver already starts. To approach the possible role of endogenous glucocorticoids in the process of regeneration we measured the changes in the expression of steroid glucocorticoid receptor gene after various regeneration intervals. After partial hepatectomy, between 0.5—168 hours from the surgery, the gene expression (mRNA) of glucocorticoid receptor was determined by reverse transcription followed by PCR and normalized to that of glycerolphoshate dehydrogenase. Two peaks of glucocorticoid receptor mRNA were detected first, between 3 and 6 hours (first peak) and a second between 24 and 36 hours. Immunoreactive glucocorticoid receptor was detected by immunohistochemistry using monoclonal anti-glucocorticoid receptor. Three days after the surgery immunohistochemical studies showed substantially more immunoreactive GcR protein in the regenerated liver than in the controls. These semiquantitative data provide evidence suggesting elevation of glucocorticoid receptor expression during regeneration of liver at mRNA and protein levels. Copyright 1999 W.B. Saunders Company Ltd on behalf of the Arányi Lajos Foundation
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ISSN:1219-4956
1532-2807
DOI:10.1053/paor.1999.0168