Sperm quality and oxidative status as affected by homogenization of liquid-stored boar semen diluted in short- and long-term extenders

Sperm quality and oxidative status as affected by homogenization of liquid-stored boar semen diluted in short- and long-term extenders

•Storage of boar semen under homogenization affects sperm quality.•Effects of homogenization occur for short- or long-term semen extenders.•Oxidative stress is unlikely to be a relevant factor of the homogenization process. Homogenization of diluted boar semen during storage has for a long time been...

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Published in:Animal reproduction science Vol. 179; pp. 67 - 79
Main Authors: Menegat, Mariana B., Mellagi, Ana Paula G., Bortolin, Rafael C., Menezes, Tila A., Vargas, Amanda R., Bernardi, Mari Lourdes, Wentz, Ivo, Gelain, Daniel P., Moreira, José Cláudio F., Bortolozzo, Fernando P.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-04-2017
Subjects:
Agitation
Animals
Hydrogen-Ion Concentration
Lipid Peroxidation
Liquid-storage
Male
Oxidative Stress
Rotation
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Specimen Handling
Swine - physiology
Swine reproduction
Temperature
Oxidative stress
Agitation
Swine reproduction
Rotation
Liquid-storage
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Summary:•Storage of boar semen under homogenization affects sperm quality.•Effects of homogenization occur for short- or long-term semen extenders.•Oxidative stress is unlikely to be a relevant factor of the homogenization process. Homogenization of diluted boar semen during storage has for a long time been regarded as beneficial. Recent studies indicated an adverse effect of homogenization on sperm quality for yet unknown reasons. This study aimed to verify the effect of homogenization on sperm parameters and to elucidate the impact of oxidative stress. Twenty-one normospermic ejaculates (21 boars) were diluted with Androstar® Plus (AND) and Beltsville Thawing Solution (BTS). Semen doses were submitted to no-homogenization (NoHom) or twice-a-day manual homogenization (2xHom) during storage at 17°C for 168h. NoHom and 2xHom were similar (P>0.05) for both short- and long-term extenders with respect to motility and kinematics parameters (CASA system), membrane viability (SYBR-14/PI), acrosome integrity, lipid peroxidation, protein oxidation, intracellular reactive oxygen species, sulfhydryl content, and total radical-trapping antioxidant potential. 2xHom reduced sperm motility and motion kinematics (VCL, VSL, VAP, BCF, and ALH) following the thermoresistance test and presented with a slight increase in pH along the storage (P=0.05) as compared to NoHom. Furthermore, 2xHom semen doses presented with a constant SOD and GSH-Px activity during storage whereas enzymatic activity increased for NoHom at the end of the storage. These findings confirm that homogenization of semen doses is detrimental to sperm quality. Moreover, it is shown that the effect of homogenization is unlikely to be primarily related to oxidative stress. Homogenization is not recommended for storage of liquid boar semen for up to 168h in both short- and long-term extenders.
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ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2017.02.003