Automated Fiber Optic Biosensor for Multiplexed Immunoassays

The multianalyte capability of the RAPTOR, a rapid, automatic, and portable fiber optic fluorimeter, was demonstrated. Employing evanescent wave illumination on polystyrene fiber optic waveguides, the RAPTOR performed fluorescent immunoassays for Bacillus globigii spores, ovalbumin, Erwinia herbicol...

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Bibliographic Details
Published in:	Environmental science & technology Vol. 34; no. 13; pp. 2845 - 2850
Main Authors:	King, Keeley D, Vanniere, Jessica M, LeBlanc, Jennifer L, Bullock, Karen E, Anderson, George P
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 01-07-2000
Subjects:	Antigens Bacillus globigii Bioassay Biochemistry Biological and medical sciences Biosensors Biotechnology Erwinia herbicola Fiber optic sensors Fiber optics Fluorometers Fundamental and applied biological sciences. Psychology Immunoassay Methods. Procedures. Technologies Polystyrenes RAPTOR Sensors Various methods and equipments Ovalbumin Phage MS2 Fluorescence Pantoea agglomerans Bacillaceae Immunosensor Immunological method Virus Portable Bacillales Levivirus Biosensor Bacteria Leviviridae Detection Phage Enterobacteriaceae Automatic Optical fiber
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Summary:	The multianalyte capability of the RAPTOR, a rapid, automatic, and portable fiber optic fluorimeter, was demonstrated. Employing evanescent wave illumination on polystyrene fiber optic waveguides, the RAPTOR performed fluorescent immunoassays for Bacillus globigii spores, ovalbumin, Erwinia herbicola, and MS2 coliphage. During a 4-day laboratory trial assaying 144 blind samples, the RAPTOR demonstrated detection of 105 cfu/mL B. globigii, 107 cfu/mL E. herbicola, and 109 pfu/mL MS2; ovalbumin detection was less favorable than expected due to sample degradation. Assays were completed in 10 min with no sample preprocessing. No false positives were identified. Antigen carryover between coupons was examined but was not found to elicit a notable response for any analytes, except B. globigii. Finally, assay results obtained after reagent and waveguides had completed 30 negative (buffer) cycles were compared with standard assay results achieved with fresh reagent and waveguides to determine whether antigen detection would decrease using cycled reagent or optical probes. Detection efficacy proved to be unaffected by the use of cycled versus fresh probes or reagent.
Bibliography:	istex:72EE6B5DBF8E4615D890570B2E5A43C9EB27018B ark:/67375/TPS-0XSQ6FXJ-5 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0013-936X 1520-5851
DOI:	10.1021/es9913535