Search Results - "Vandenheede, Isabel"

Modern chromatographic and mass spectrometric techniques for protein biopharmaceutical characterization by Sandra, Koen, Vandenheede, Isabel, Sandra, Pat

“…•Protein biopharmaceuticals are highly complex and heterogeneous macromolecules.•Chromatography and MS are at the forefront in protein…”
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The versatility of heart-cutting and comprehensive two-dimensional liquid chromatography in monoclonal antibody clone selection by Sandra, Koen, Steenbeke, Mieke, Vandenheede, Isabel, Vanhoenacker, Gerd, Sandra, Pat

“…•Protein A affinity chromatography was combined with SEC, CEX or RPLC–MS in a heart-cutting 2D set-up.•Attributes such as mAb titer and structure can…”
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Comprehensive two-dimensional liquid chromatography of therapeutic monoclonal antibody digests by Vanhoenacker, Gerd, Vandenheede, Isabel, David, Frank, Sandra, Pat, Sandra, Koen

“…Comprehensive two-dimensional liquid chromatography (LC×LC) is here proposed as a novel tool for peptide mapping of therapeutic monoclonal antibodies in both…”
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Multiple heart-cutting and comprehensive two-dimensional liquid chromatography hyphenated to mass spectrometry for the characterization of the antibody-drug conjugate ado-trastuzumab emtansine by Sandra, Koen, Vanhoenacker, Gerd, Vandenheede, Isabel, Steenbeke, Mieke, Joseph, Maureen, Sandra, Pat

“…•2D-LC-MS was applied to unravel the complexity of the antibody-drug conjugate ado-trastuzumab emtansine.•mLC-LC was applied on the intact drug and fragments…”
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2D-CEX–FcRn–MS to Study Structure/Function Relation of mAb Charge Variants by Verscheure, Liesa, Vandenheede, Isabel, De Rore, Eline, Meersseman, Mabelle, Hanssens, Valerie, Meerschaert, Kris, Stals, Hilde, Sandra, Pat, Lynen, Frederic, Borgions, Filip, Sandra, Koen

“…The automated elucidation of the interplay between monoclonal antibody (mAb) structure and function using two-dimensional liquid chromatography–mass…”
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Characterization of mAb size heterogeneity originating from a cysteine to tyrosine substitution using denaturing and native LC-MS by Ruppen, Isabel, Verscheure, Liesa, Vandenheede, Isabel, Ortiz, Alexia, de Melo, Iván Sánchez, Liebig, Timo, Sandra, Pat, Beydon, Marie-Elise, Sandra, Koen

“…Upon assessing the comparability between a biosimilar mAb and its reference product by non-reducing CE-SDS, increased levels of a heavy-heavy-light chain (HHL)…”
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Similarity demonstrated between isolated charge variants of MB02, a biosimilar of bevacizumab, and Avastin® following extended physicochemical and functional characterization by Ruppen, Isabel, Beydon, Marie-Elise, Solís, Clea, Sacristán, Daniel, Vandenheede, Isabel, Ortiz, Alexia, Sandra, Koen, Adhikary, Laxmi

“…The majority of recombinant mAb products contain heterogeneous charge variants, commonly the result of post-translational modifications occurring during cell…”
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Similarity demonstrated between isolated charge variants of MB02, a biosimilar of bevacizumab, and Avastin® following extended physicochemical and functional characterization by Ruppen, Isabel, Beydon, Marie-Elise, Solís, Clea, Sacristán, Daniel, Vandenheede, Isabel, Ortiz, Alexia, Sandra, Koen, Adhikary, Laxmi

“…The majority of recombinant mAb products contain heterogeneous charge variants, commonly the result of post-translational modifications occurring during cell…”
Get full text